Conboy James P, Istúriz Petitjean Irene, van der Net Anouk, Koenderink Gijsje H
Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2629 HZ Delft, The Netherlands.
Biophys Rev (Melville). 2024 Jun 3;5(2):021307. doi: 10.1063/5.0198119. eCollection 2024 Jun.
Cell migration is a fundamental process for life and is highly dependent on the dynamical and mechanical properties of the cytoskeleton. Intensive physical and biochemical crosstalk among actin, microtubules, and intermediate filaments ensures their coordination to facilitate and enable migration. In this review, we discuss the different mechanical aspects that govern cell migration and provide, for each mechanical aspect, a novel perspective by juxtaposing two complementary approaches to the biophysical study of cytoskeletal crosstalk: live-cell studies (often referred to as top-down studies) and cell-free studies (often referred to as bottom-up studies). We summarize the main findings from both experimental approaches, and we provide our perspective on bridging the two perspectives to address the open questions of how cytoskeletal crosstalk governs cell migration and makes cells move.
细胞迁移是生命的一个基本过程，高度依赖于细胞骨架的动态和机械特性。肌动蛋白、微管和中间丝之间密集的物理和生化相互作用确保了它们的协调，以促进并实现细胞迁移。在本综述中，我们讨论了控制细胞迁移的不同力学方面，并针对每个力学方面，通过将两种互补的细胞骨架相互作用生物物理研究方法并列，提供了一个新颖的视角：活细胞研究（通常称为自上而下的研究）和无细胞研究（通常称为自下而上的研究）。我们总结了这两种实验方法的主要发现，并就如何弥合这两种观点以解决细胞骨架相互作用如何控制细胞迁移以及使细胞移动这一开放性问题给出了我们的观点。
