Department of Instrumentation and Analytical Chemistry, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China.
Department of Instrumentation and Analytical Chemistry, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, PR China; Technology Innovation Center of Food Safety Technique of Inspection for State Market Regulation (Rapid Screening and Traceability for Edible Agricultural Product Safety), PR China.
J Chromatogr A. 2024 Aug 16;1729:465036. doi: 10.1016/j.chroma.2024.465036. Epub 2024 May 30.
In this work, a microchip gas chromatography (GC) column assembly utilizing a three-dimensional (3D) printed micro oven and a flexible stainless steel capillary column was developed. The assembly's performance and separation capabilities were characterized. The key components include a 3D printed aluminum plate (7.50 × 7.50 × 0.16 cm) with a 3-meter-long circular spiral channel, serving as the oven, and the column coiled on the channel with an inner diameter of 320 μm and a stationary phase of OV-1. A heating ceramic plate was affixed on the opposite side of the plate. The assembly weighed 40.3 g. The design allows for easy disassembly, or stacking of heating devices and columns, enabling flexibility in adjusting column length. When using n-C as the test analyte at 140 °C, a retention factor (k) was 8.5, and 7797 plates (2599 plates/m) were obtained. The assembly, employing resistance heating, demonstrated effective separation performance for samples containing alkanes, aromatics, alcohols and ketones, with good reproducibility. The reduction in theoretical plates compared to oven heating was only 2.95 %. In the boiling point range of C to C, rapid temperature programming (120 °C/min) was achieved with a power consumption of 119.512 W. The assembly was successfully employed to separate benzene series compounds, gasoline and volatile organic compounds (VOCs), demonstrating excellent separation performance. This innovative design addresses the challenges of the complexity and low repeatability of the fabrication process and the high cost associated with microchip columns. Furthermore, its versatility makes it suitable for outdoor analysis applications.
在这项工作中，开发了一种利用三维（3D）打印微烤箱和柔性不锈钢毛细管柱的微芯片气相色谱（GC）柱组件。对组件的性能和分离能力进行了表征。关键组件包括一个 3D 打印的铝板（7.50×7.50×0.16 厘米），其带有一个 3 米长的圆形螺旋通道，用作烤箱，以及缠绕在通道上的柱，其内径为 320μm，固定相为 OV-1。在板的对面附有加热陶瓷板。组件重 40.3 克。该设计允许轻松拆卸或堆叠加热装置和柱子，从而灵活调整柱子长度。当使用 n-C 作为 140°C 的测试分析物时，保留因子（k）为 8.5，获得了 7797 个板（2599 个板/m）。该组件采用电阻加热，对包含烷烃、芳烃、醇和酮的样品表现出有效的分离性能，具有良好的重现性。与烤箱加热相比，理论板数的减少仅为 2.95%。在沸点范围为 C 到 C 的情况下，实现了快速程序升温（120°C/min），功率消耗为 119.512 W。该组件成功用于分离苯系化合物、汽油和挥发性有机化合物（VOCs），表现出优异的分离性能。这种创新设计解决了微芯片柱制造过程的复杂性和低重复性以及高成本的挑战。此外，其多功能性使其适用于户外分析应用。
