Jiang Shi-Die, Chen Yu-Ang, Guo Peng-Yu, Sun Jun, Feng Hua-Yue, Li Yi, Wang Jin-Jun, Wei Dan-Dan
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Insect Sci. 2025 Apr;32(2):437-456. doi: 10.1111/1744-7917.13387. Epub 2024 Jun 6.
Psocodean species are emerging as significant sanitary and stored-product pests, posing threats to human health and global food security. Out of an estimated 10 000 species, the whole genome sequences of only 4 species have been published. Genomic resources are crucial for establishing effective pest control and enhancing our understanding of the evolution of psocodean species. In this study, we employed Illumina and PacBio sequencing along with Hi-C scaffolding techniques to generate a chromosome-level genome assembly for the parthenogenetic booklouse Liposcelis bostrychophila. The assembled genome of this booklouse measures 291.67 Mb in length and comprises 9 chromosomes. Notably, the genome of L. bostrychophila exhibits a high level of heterozygosity and features a distinctive nonhomologous chromosome. This heterozygous characteristic of the parthenogenetic booklouse genome may arise from high mutation rates, based on genomic variations analysis across multiple generations. Our analysis revealed significantly expanded gene families, primarily associated with the detoxification and feeding habits of L. bostrychophila. These include integument esterases (ESTs), ATP-binding cassette (ABC) transporter genes and gustatory receptors (GRs). The high-quality genome sequence of L. bostrychophila provides valuable resources for further study on the molecular mechanisms of stress resistance. It enables researchers to identify crucial functional genes and facilitates research on the population genetics, evolution and phylogeny of booklice.
啮虫目昆虫正逐渐成为重要的卫生害虫和仓储害虫，对人类健康和全球粮食安全构成威胁。在估计的10000种啮虫目昆虫中，仅4种的全基因组序列已被公布。基因组资源对于建立有效的害虫防治方法以及增进我们对啮虫目昆虫进化的理解至关重要。在本研究中，我们采用了Illumina和PacBio测序技术以及Hi-C支架技术，为孤雌生殖的嗜卷书虱生成了染色体水平的基因组组装。这种书虱的组装基因组长度为291.67 Mb，由9条染色体组成。值得注意的是，嗜卷书虱的基因组表现出高度杂合性，并具有一条独特的非同源染色体。基于多代的基因组变异分析，这种孤雌生殖书虱基因组的杂合特性可能源于高突变率。我们的分析揭示了显著扩张的基因家族，主要与嗜卷书虱的解毒和取食习性相关。这些基因包括体表酯酶（ESTs）、ATP结合盒（ABC）转运蛋白基因和味觉受体（GRs）。嗜卷书虱的高质量基因组序列为进一步研究其抗逆分子机制提供了宝贵资源。它使研究人员能够鉴定关键功能基因，并促进对书虱种群遗传学、进化和系统发育的研究。
