Jiang Shi-Die, Chen Yu-Ang, Sun Sheng-Yuan, Smagghe Guy, Wang Jin-Jun, Wei Dan-Dan
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, China.
Department of Plants and Crops, Ghent University, Ghent, Belgium.
Sci Data. 2024 Dec 2;11(1):1310. doi: 10.1038/s41597-024-04179-y.
Liposcelis tricolor (Psocoptera: Liposcelididae) is a significant pest affecting stored products globally. However, due to the lack of a detailed genomic reference, the mechanisms of sex determination, stress resistance, and potential control methods for this booklouse remain poorly understood. In this study, the chromosome-level genome of L. tricolor was assembled by employing Illumina, Nanopore, and Hi-C sequencing technologies. The final genome size was determined to be 229.33 Mb, anchored to 9 pseudo-chromosomes. BUSCO analysis showed that 99.2% of complete BUSCOs were identified, suggesting the high completeness of the genome. A total of 91.49 Mb of repetitive sequences, accounting for 38.84% of the total genome, were annotated, and 15,647 protein-coding genes were predicted, with 88.17% functionally annotated. Additionally, we identified 25 typical sex-determining genes based on the genomic data. This high-quality genome assembly provides a crucial foundation for advancing our comprehension of the molecular biology, genetics, and potential control strategies for psocid L. tricolor.
三色书虱（啮目：书虱科）是一种在全球范围内影响储存产品的重要害虫。然而，由于缺乏详细的基因组参考，这种书虱的性别决定、抗逆机制及潜在防治方法仍知之甚少。在本研究中，利用Illumina、Nanopore和Hi-C测序技术组装了三色书虱的染色体水平基因组。最终确定基因组大小为229.33 Mb，锚定到9条假染色体上。BUSCO分析表明，99.2%的完整BUSCO被鉴定出来，表明基因组的完整性很高。共注释了91.49 Mb的重复序列，占基因组总量的38.84%，预测了15,647个蛋白质编码基因，其中88.17%具有功能注释。此外，我们基于基因组数据鉴定出25个典型的性别决定基因。这种高质量的基因组组装为增进我们对三色书虱分子生物学、遗传学及潜在防治策略的理解提供了关键基础。
