School of Environmental and Chemical Engineering, Shenyang University of Technology, Shenyang, 110870, China.
Pharmaron Beijing Co., Ltd., Beijing, 100176, China.
Biotechnol Lett. 2024 Oct;46(5):767-780. doi: 10.1007/s10529-024-03497-w. Epub 2024 Jun 6.
Chlorimuron-ethyl is currently the primary herbicide used for chemical weed control in a soybean field. In this study, a solid microbial inoculum (corn stalk-white rot fungus (W-1)) was prepared for the remediation of farmland soil contaminated by chlorimuron-ethyl. Firstly, the preparation method of the microbial inoculum was studied. Secondly, the degradation rate of the chlorimuron-ethyl in the ground by the solid microbial inoculum is improved by optimizing the proportion of the protective agent. Then the effects of applying solid microbial inoculum, free bacteria and corn straw on the degradation rate of chlorimuron-ethyl in soil were weighed. Finally, Illumina MiSeq sequencing was used to measure the composition and diversity of bacterial and fungal communities in the ground before and after using microbial inoculum. The degradation rate of chlorimuron-ethyl in soil by solid microbial inoculum was 84.87% after 20 d using corn straw as the support, room temperature drying, 4% Ca(PO) as the protective drying agent, and 1%(w) dextrin as the ultraviolet protective agent. Inoculation of white rot fungi could significantly affect the community structure of bacteria and fungi in the soil, making the chlorimuron-ethyl degrading communities become the dominant communities and playing an essential role in the degradation of chlorimuron-ethyl. The results showed that using solid microbial inoculum was an effective way to repair farmland soil polluted by chlorimuron-ethyl.
氯嘧磺隆-乙酯目前是大豆田中用于化学除草的主要除草剂。在这项研究中，制备了一种固体微生物接种剂（玉米秸秆-白腐真菌（W-1）），用于修复农田土壤中被氯嘧磺隆-乙酯污染的土地。首先，研究了微生物接种剂的制备方法。其次，通过优化保护剂的比例，提高了固体微生物接种剂对土壤中氯嘧磺隆-乙酯的降解率。然后，衡量了固体微生物接种剂、游离细菌和玉米秸秆对土壤中氯嘧磺隆-乙酯降解率的影响。最后，使用 Illumina MiSeq 测序测量了使用微生物接种剂前后土壤中细菌和真菌群落的组成和多样性。以玉米秸秆为载体，室温干燥，4%Ca(PO)作为保护干燥剂，1%（w）糊精作为紫外线保护剂，固体微生物接种剂在 20 d 后，土壤中氯嘧磺隆-乙酯的降解率为 84.87%。接种白腐真菌可以显著影响土壤中细菌和真菌的群落结构，使氯嘧磺隆-乙酯降解群落成为优势群落，在氯嘧磺隆-乙酯的降解中发挥重要作用。结果表明，使用固体微生物接种剂是修复氯嘧磺隆-乙酯污染农田土壤的有效方法。
