Fujian Forestry Vocational Technical College, Nanping, 353000, China.
Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute Chinese Academy of Agricultural Sciences, 9 South Meiling Road, Hangzhou, 310008, China.
Food Environ Virol. 2024 Sep;16(3):409-421. doi: 10.1007/s12560-024-09604-4. Epub 2024 Jun 6.
This study focused on the identification of rot-causing fungi in Citrus × tangelo (tangelo) with a particular emphasis on investigating the inhibitory effects of acidic electrolyzed water on the identified pathogens. The dominant strains responsible for postharvest decay were isolated from infected tangelo fruits and characterized through morphological observation, molecular identification, and pathogenicity detection. Two strains were isolated from postharvest diseased tangelo fruits, cultured and morphologically characterized, and had their gene fragments amplified using primers ITS1 and ITS4. The results revealed the rDNA-ITS sequence of two dominant pathogens were 100% homologous with those of Penicillium citrinum and Aspergillus sydowii. These isolated fungi were confirmed to induce tangelo disease, and subsequent re-isolation validated their consistency with the inoculum. Antifungal tests demonstrated that acidic electrolyzed water (AEW) exhibited a potent inhibitory effect on P. citrinum and A. sydowii, with EC values of 85.4 μg/mL and 60.12 μg/mL, respectively. The inhibition zones of 150 μg/mL AEW to 2 kinds of pathogenic fungi were over 75 mm in diameter. Furthermore, treatment with AEW resulted in morphological changes such as bending and shrinking of the fungal hyphae surface. In addition, extracellular pH, conductivity, and absorbance at 260 nm of the fungi hypha significantly increased post-treatment with AEW. Pathogenic morphology and IST sequencing analysis confirmed P. citrinum and A. sydowii as the primary pathogenic fungi, with their growth effectively inhibited by AEW.
本研究聚焦于鉴定导致柑橘属宽皮橘与甜橙杂种（橘柚）腐烂的真菌，特别关注酸性电解水对鉴定出的病原体的抑制作用。从感染的橘柚果实中分离出导致采后腐烂的优势菌株，通过形态观察、分子鉴定和致病性检测进行特征描述。从采后患病的橘柚果实中分离出两株优势菌株，进行培养和形态特征描述，并使用 ITS1 和 ITS4 引物扩增其基因片段。结果表明，两种主要病原菌的 rDNA-ITS 序列与青霉和枝孢霉的序列完全同源。这些分离出的真菌被证实会引起橘柚病害，随后的再分离验证了它们与接种物的一致性。抑菌试验表明，酸性电解水（AEW）对青霉和枝孢霉表现出强烈的抑制作用，其 EC 值分别为 85.4μg/mL 和 60.12μg/mL。150μg/mL 的 AEW 对 2 种病原菌的抑菌圈直径均超过 75mm。此外，AEW 处理导致真菌菌丝表面弯曲和收缩等形态变化。此外，AEW 处理后真菌菌丝的胞外 pH 值、电导率和 260nm 吸光度显著增加。致病性形态和 IST 测序分析证实青霉和枝孢霉是主要的病原菌，AEW 能有效抑制它们的生长。
