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植物乳杆菌 PA21 的基因组挖掘：对其抗菌潜力的深入了解。

Genome mining of Lactiplantibacillus plantarum PA21: insights into its antimicrobial potential.

机构信息

Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia (UPM), Serdang, 43400, Selangor, Malaysia.

Halal Products Research Institute, Universiti Putra Malaysia (UPM), Serdang, 43400, Selangor, Malaysia.

出版信息

BMC Genomics. 2024 Jun 6;25(1):571. doi: 10.1186/s12864-024-10451-7.

DOI:10.1186/s12864-024-10451-7
PMID:38844835
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11157852/
Abstract

BACKGROUND

The dramatic increase of antimicrobial resistance in the healthcare realm has become inexorably linked to the abuse of antibiotics over the years. Therefore, this study seeks to identify potential postbiotic metabolites derived from lactic acid bacteria such as Lactiplantibacillus plantarum that could exhibit antimicrobial properties against multi-drug resistant pathogens.

RESULTS

In the present work, the genome sequence of Lactiplantibacillus plantarum PA21 consisting of three contigs was assembled to a size of 3,218,706 bp. Phylogenomic analysis and average nucleotide identity (ANI) revealed L. plantarum PA21 is closely related to genomes isolated from diverse niches such as dairy products, food, and animals. Genome mining through the BAGEL4 and antiSMASH database revealed four bacteriocins in a single cluster and four regions of biosynthetic gene clusters responsible for the production of bioactive compounds. The potential probiotic genes indirectly responsible for postbiotic metabolites production were also identified. Additionally, in vitro studies showed that the L. plantarum PA21 cell-free supernatant exhibited antimicrobial activity against all nine methicillin-resistant Staphylococcus aureus (MRSA) and three out of 13 Klebsiella pneumoniae clinical isolates tested.

CONCLUSION

Results in this study demonstrates that L. plantarum PA21 postbiotic metabolites is a prolific source of antimicrobials against multi-drug resistant pathogens with potential antimicrobial properties.

摘要

背景

近年来，抗生素滥用与医疗保健领域中抗菌药物耐药性的急剧增加息息相关。因此，本研究旨在寻找潜在的后生元代谢产物，这些代谢产物可能来自于植物乳杆菌等乳酸菌，具有抗多种耐药性病原体的抗菌特性。

结果

在本工作中，组装了由三个连续体组成的植物乳杆菌 PA21 的基因组序列，大小为 3218706bp。系统发育分析和平均核苷酸同一性（ANI）表明，L. plantarum PA21 与从乳制品、食品和动物等多种生态位分离的基因组密切相关。通过 BAGEL4 和 antiSMASH 数据库进行基因组挖掘，发现单个簇中有四种细菌素和四个负责生物活性化合物生产的生物合成基因簇区域。还鉴定了间接负责后生元代谢产物产生的潜在益生菌基因。此外，体外研究表明，植物乳杆菌 PA21 的无细胞上清液对所有 9 株耐甲氧西林金黄色葡萄球菌（MRSA）和 13 株肺炎克雷伯菌临床分离株中的 3 株均表现出抗菌活性。

结论

本研究结果表明，植物乳杆菌 PA21 的后生元代谢产物是一种对抗多种耐药性病原体的抗菌剂的丰富来源，具有潜在的抗菌特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/80d47ac861fb/12864_2024_10451_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/c03a68a9de4e/12864_2024_10451_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/d129f81a3c58/12864_2024_10451_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/ad16afb6e68f/12864_2024_10451_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/52ae8861d1a1/12864_2024_10451_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/80d47ac861fb/12864_2024_10451_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/c03a68a9de4e/12864_2024_10451_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/d129f81a3c58/12864_2024_10451_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/ad16afb6e68f/12864_2024_10451_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/52ae8861d1a1/12864_2024_10451_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4b22/11157852/80d47ac861fb/12864_2024_10451_Fig5_HTML.jpg

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本文引用的文献

1
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2
Automated analysis of genomic sequences facilitates high-throughput and comprehensive description of bacteria.基因组序列的自动化分析有助于对细菌进行高通量和全面的描述。
ISME Commun. 2021 May 20;1(1):16. doi: 10.1038/s43705-021-00017-z.
3
Whole genome sequence data of IMI 507027.IMI 507027的全基因组序列数据。
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Front Microbiol. 2024 Sep 11;15:1436378. doi: 10.3389/fmicb.2024.1436378. eCollection 2024.
Data Brief. 2022 Mar 6;42:108025. doi: 10.1016/j.dib.2022.108025. eCollection 2022 Jun.
4
Comparative genomic analysis of 455 Lactiplantibacillus plantarum isolates: Habitat-specific genomes shaped by frequent recombination.455 株植物乳杆菌的比较基因组分析：由频繁重组形成的具有栖息地特异性的基因组。
Food Microbiol. 2022 Jun;104:103989. doi: 10.1016/j.fm.2022.103989. Epub 2022 Jan 16.
5
Potential Adjuvant Therapeutic Effect of Probio-88 Postbiotics against SARS-COV-2.Probio-88后生元对新型冠状病毒的潜在辅助治疗作用
Vaccines (Basel). 2021 Sep 24;9(10):1067. doi: 10.3390/vaccines9101067.
6
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Food Res Int. 2021 Oct;148:110595. doi: 10.1016/j.foodres.2021.110595. Epub 2021 Jul 10.
7
Comprehensive pan-genome analysis of Lactiplantibacillus plantarum complete genomes.全面泛基因组分析植物乳杆菌完整基因组。
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8
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9
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Nat Rev Gastroenterol Hepatol. 2021 Sep;18(9):649-667. doi: 10.1038/s41575-021-00440-6. Epub 2021 May 4.