Colić M, Matanović D, Hegedis L, Dujić A
Institute for Experimental Medicine, Military Medical Academy, Belgrade, Yugoslavia.
Immunology. 1988 Oct;65(2):277-84.
Molecular microenvironmental heterogeneity within rat thymus was studied by a panel of 13 monoclonal antibodies (mAbs) raised to thymic epithelial (TE) cells and mesenchymal stroma. Based on their anatomical distribution patterns observed with immunohistological techniques on frozen sections and double immunostaining using anti-keratin antibodies to identify epithelium, they were subdivided into five groups: (i) pan TE cells antibodies (R-MC 2, 3 and 8); (ii) cortical TE cells antibodies (R-MC 13-17); (iii) antibodies detecting subcapsular and subtrabecular TE cells and most medullary TE cells (R-MC 18-20); (iv) antibody to Hassall's corpuscles (HC) and a small subpopulation of medullary TE cells (R-MC 22); (v) mesenchymal stroma antibody (R-MC 23). The obtained results show phenotypic heterogeneity of rat thymic epithelium and its distinction compared to mesodermal-derived compartment.
利用一组针对胸腺上皮（TE）细胞和间充质基质产生的13种单克隆抗体（mAb），研究了大鼠胸腺内的分子微环境异质性。根据在冰冻切片上通过免疫组织学技术观察到的解剖分布模式，以及使用抗角蛋白抗体鉴定上皮的双重免疫染色，将它们分为五组：（i）全TE细胞抗体（R-MC 2、3和8）；（ii）皮质TE细胞抗体（R-MC 13 - 17）；（iii）检测被膜下和小梁下TE细胞以及大多数髓质TE细胞的抗体（R-MC 18 - 20）；（iv）针对哈氏小体（HC）和一小部分髓质TE细胞的抗体（R-MC 22）；（v）间充质基质抗体（R-MC 23）。获得的结果显示了大鼠胸腺上皮的表型异质性及其与中胚层来源区室的区别。
