Haller I
J Antimicrob Chemother. 1985 Jan;15 Suppl A:197-9. doi: 10.1093/jac/15.suppl_a.197.
Commercial tissue culture chambers were modified in such a way that they contained two portions of medium separated by a horizontal membrane. When grown on this membrane, Vero (monkey kidney) cells formed continuous cell monolayers. By comparing the penetration of antibiotics through monolayer-covered and cell-free membranes, the retention brought about by the cell layer could be measured. Only minor differences were seen between oxacillin, mezlocillin and ciprofloxacin. Penetration of polymyxin B through the cell layer proved to be reduced by about 40% in comparison to oxacillin.
对商用组织培养箱进行了改装，使其包含由水平膜分隔的两部分培养基。当在这种膜上生长时，Vero（猴肾）细胞形成连续的细胞单层。通过比较抗生素在覆盖有单层细胞的膜和无细胞的膜中的渗透情况，可以测量细胞层所造成的滞留。苯唑西林、美洛西林和环丙沙星之间仅观察到微小差异。与苯唑西林相比，多粘菌素B通过细胞层的渗透被证明降低了约40%。
