Adam D, Wilhelm K, Chyský V
Arzneimittelforschung. 1981;31(11):1972-6.
Blood present in the tissue distorts the results obtained during measurement of antibiotic tissue levels. The greater the blood supply to the tissue, the bigger the error when tissue levels are measured. The examination of tissue removed during temporary intraoperative ischemia is an alternative to the indirect mathematical method of correcting errors of blood level measurement. This technique was tested by determining tissue concentrations after i.v. injection of 4 g mezlocillin and 2 g oxacillin. The tissue was removed during operations on limbs after the application of an Esmarch's bandage. Determination of hemoglobin in ischemic tissue confirmed exsanguination so that the 6-((R)-2-[3-methylsulfonyl-2-oxo-imidazolidine-1-carboxamido]-2-phenylacetamido )-penicillanic acid-Na (mezlocillin) and 5-methyl-3-phenyl-4-isoxazolylpenicillin (oxacillin) concentrations determined corresponded to the actual tissue levels. The concentrations of mezlocillin and oxacillin in fat, skin, tendon, muscle, ganglion, aponeurosis, tumour, ligament, subcutis, synovial fluid, epineurium and serum are given in the text.
组织中的血液会干扰抗生素组织水平测量所获得的结果。组织的血液供应越丰富，测量组织水平时的误差就越大。对术中暂时缺血期间切除的组织进行检查是一种替代血液水平测量误差间接数学校正方法的手段。通过静脉注射4g美洛西林和2g苯唑西林后测定组织浓度来测试该技术。在使用埃斯马赫绷带后对四肢进行手术时切除组织。对缺血组织中的血红蛋白进行测定证实已排尽血液，因此所测定的6-((R)-2-[3-甲基磺酰基-2-氧代-咪唑烷-1-甲酰胺基]-2-苯乙酰氨基)-青霉烷酸-Na（美洛西林）和5-甲基-3-苯基-4-异恶唑基青霉素（苯唑西林）浓度与实际组织水平相符。文中给出了美洛西林和苯唑西林在脂肪、皮肤、肌腱、肌肉、神经节、腱膜、肿瘤、韧带、皮下组织、滑液、神经外膜和血清中的浓度。
