Teerarak Montinee, Pilasombut Komkhae, Laosinwattana Chamroon
Department of Plant Production Technology, School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Office of Administrative Interdisciplinary Program on Agricultural Technology, School of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Heliyon. 2024 May 22;10(11):e31636. doi: 10.1016/j.heliyon.2024.e31636. eCollection 2024 Jun 15.
To extend the vase life of cut flowers, there is now a trend of using plant essential oils in place of synthetic chemicals, as they are fully biodegradable, more eco-friendly, and safer. The objective of this study was to examine the possible application and postharvest quality effects of three plant essential oils namely, ginger ( Roscoe), peppermint ( L.), and citronella ( Rendle), as natural vase solution for cut flowers. Peppermint essential oil showed promise as a holding solution for extending the vase life of orchids. To confirm vase life extension, emulsions containing peppermint essential oil at concentrations of 50 and 100 μg mL combined with 4 % glucose to formulate holding solutions applied to orchids. Vase life, some biochemical changes, electrolyte leakage, total microbial count in the holding solution, and physical condition via scanning electron microscopy (SEM) were evaluated over a period of 25 days. The three major compounds in peppermint essential oil were identified as menthol (33.24 %), 1-menthone (18.91 %) and menthofuran (14.85 %). The essential oil was applied in emulsion form as a holding solution. Treatment with 4 % glucose and either 50 or 100 μg mL peppermint essential oil prolonged the vase life of orchids to up to 28 days. Scanning electron microscopy on Day 7 showed that the xylem vessels of treated orchids remained clear, suggesting reduced microbial plugging at the stalk end. Similarly, on Day 20, a reduced microbial cell count was observed for treated orchids (<1 log CFU mL) in comparison with controls (7.20 ± 0.04 log CFU mL). Finally, the essential oil improved flower quality by helping preserve petal membrane stability and petal anthocyanin content. Our results suggest the application of peppermint essential oil as a novel alternative to chemicals used in holding solutions for extending the vase life of orchids.
为延长切花的瓶插寿命,目前有一种使用植物精油替代合成化学品的趋势,因为植物精油完全可生物降解、更环保且更安全。本研究的目的是考察三种植物精油,即姜(Roscoe)、薄荷(L.)和香茅(Rendle)作为切花瓶插天然溶液的可能应用及对采后品质的影响。薄荷精油有望作为延长兰花瓶插寿命的保鲜液。为证实瓶插寿命的延长,将浓度为50和100μg/mL的薄荷精油与4%葡萄糖混合配制成保鲜液乳液用于处理兰花。在25天的时间里评估了瓶插寿命、一些生化变化、电解质渗漏、保鲜液中的总微生物数量以及通过扫描电子显微镜(SEM)观察的物理状况。薄荷精油中的三种主要成分被鉴定为薄荷醇(33.24%)、1-薄荷酮(18.91%)和薄荷呋喃(14.85%)。该精油以乳液形式作为保鲜液使用。用4%葡萄糖和50或100μg/mL薄荷精油处理可将兰花的瓶插寿命延长至28天。第7天的扫描电子显微镜观察表明,处理过的兰花木质部导管保持通畅,表明茎端的微生物堵塞减少。同样,在第20天,与对照(7.20±0.04 log CFU/mL)相比,处理过的兰花微生物细胞数量减少(<1 log CFU/mL)。最后,该精油通过帮助保持花瓣膜稳定性和花瓣花青素含量提高了花朵品质。我们的结果表明,薄荷精油可作为保鲜液中用于延长兰花瓶插寿命的化学品的新型替代品。