Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah, Saudi Arabia.
Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.
Front Cell Infect Microbiol. 2024 May 22;14:1375872. doi: 10.3389/fcimb.2024.1375872. eCollection 2024.
is notorious for its multidrug resistance and its involvement in hospital-acquired infections. In this study, 20 bacterial strains isolated from soil samples near the Hindan River in Ghaziabad, India, were investigated for their biochemical and morphological characteristics, with a focus on identifying strains with exceptional drug resistance and pyocyanin production.
The isolated bacterial strains were subjected to biochemical and morphological analyses to characterize their properties, with a particular emphasis on exopolysaccharide production. Strain GZB16/CEES1, exhibiting remarkable drug resistance and pyocyanin production. Biochemical and molecular analyses, including sequencing of its 16S rRNA gene (accession number LN735036.1), plasmid-curing assays, and estimation of plasmid size, were conducted to elucidate its drug resistance mechanisms and further pyocynin based target the Strain GZB16/CEES1 demonstrated 100% resistance to various antibiotics used in the investigation, with plasmid-curing assays, suggesting plasmid-based resistance gene transmission. The plasmid in GZB16/CEES1 was estimated to be approximately 24 kb in size. The study focused on 's pyocyanin production, revealing its association with anticandidal activity. The minimum inhibitory concentration (MIC) of the bacterial extract against was 50 μg/ml, with a slightly lower pyocyanin-based MIC of 38.5 μg/ml. Scanning electron microscopy illustrated direct interactions between strains and cells, leading to the destruction of the latter.
These findings underscore the potential of in understanding microbial interactions and developing strategies to combat fungal infections. The study highlights the importance of investigating bacterial-fungal interactions and the role of pyocyanin in antimicrobial activity. Further research in this area could lead to the development of novel therapeutic approaches for combating multidrug-resistant infections.
因其多药耐药性及其在医院获得性感染中的作用而臭名昭著。在这项研究中，对从印度加济阿巴德欣丹河附近土壤样本中分离出的 20 株细菌菌株进行了生化和形态特征研究，重点是鉴定具有特殊耐药性和绿脓菌素产生的菌株。
对分离出的细菌菌株进行生化和形态分析，以表征其特性，特别强调胞外多糖的产生。菌株 GZB16/CEES1 表现出显著的耐药性和绿脓菌素产生。进行了生化和分子分析，包括其 16S rRNA 基因（登录号 LN735036.1）的测序、质粒消除试验和质粒大小估计，以阐明其耐药机制，并进一步针对绿脓菌素的靶标。GZB16/CEES1 对研究中使用的各种抗生素表现出 100%的耐药性，通过质粒消除试验，提示存在基于质粒的耐药基因传递。GZB16/CEES1 中的质粒估计大小约为 24kb。该研究重点关注 的绿脓菌素产生，揭示其与抗真菌活性的关联。细菌提取物对 的最小抑菌浓度（MIC）为 50μg/ml，基于绿脓菌素的 MIC 略低，为 38.5μg/ml。扫描电子显微镜显示了 菌株与 细胞之间的直接相互作用，导致后者被破坏。
这些发现强调了 在理解微生物相互作用和开发抗真菌感染策略方面的潜力。该研究强调了研究细菌-真菌相互作用和绿脓菌素在抗菌活性中的作用的重要性。该领域的进一步研究可能导致开发针对多药耐药感染的新型治疗方法。
