Paliu Iulia-Alexandra, Bita Andrei, Diaconu Magdalena, Tica Andrei-Adrian
Department of Pharmacology, University of Medicine and Pharmacy of Craiova, Romania.
Department of Pharmacognosy, University of Medicine and Pharmacy of Craiova, Romania.
Curr Health Sci J. 2024 Jan-Mar;50(1):87-93. doi: 10.12865/CHSJ.50.01.12. Epub 2024 Mar 31.
Owing to ergosterol content, after UV irradiation yeast become a well-known source of ergocalciferol (vitamin D2). Additionally, pharmaceutical yeast-based supplements may represent a suitable option for treating hypovitaminosis, especially in patients adhering to a vegan diet. Using the high-performance liquid chromatography-ultraviolet (HPLC-UV) methodology our study sought to analyse three commercially available yeast-based vitamin D2 supplements while comparing the effect of UV-C irradiation (254 nm) on yeast biomass derived from the brewing process and pure ergosterol. The two compounds were precisely separated under the described conditions in an efficient and quick manner with a retention time (Rt) of 4.152 ± 0.018 minutes for vitamin D2 and 5.097 ± 0.013 minutes for ergosterol. However, when approaching the quantitative analysis, based on our findings, it appears that the pharmaceutical supplements deviate from the declared amount of substance indicated on the label. 15 minutes of UV-C irradiation generates vitamin D2 in yeast biomass with a conversion rate of 1.78%. Also, high content of ergosterol, beside vitamin D2 formation after irradiation, may trigger the appearance of secondary products such as tachysterol.
由于麦角固醇的含量，紫外线照射后酵母成为众所周知的麦角钙化醇（维生素D2）来源。此外，基于酵母的药物补充剂可能是治疗维生素缺乏症的合适选择，特别是对于坚持纯素饮食的患者。本研究采用高效液相色谱-紫外（HPLC-UV）方法，分析了三种市售的基于酵母的维生素D2补充剂，同时比较了UV-C照射（254nm）对酿造过程中产生的酵母生物质和纯麦角固醇的影响。在所述条件下，两种化合物以高效、快速的方式精确分离，维生素D2的保留时间（Rt）为4.152±0.018分钟，麦角固醇的保留时间为5.097±0.013分钟。然而，在进行定量分析时，根据我们的研究结果，药物补充剂似乎与标签上标明的物质申报量存在偏差。15分钟的UV-C照射在酵母生物质中产生维生素D2，转化率为1.78%。此外，除了照射后形成维生素D2外，高含量的麦角固醇可能会引发诸如速甾醇等副产物的出现。
