Cho Tae Joon, Reipa Vytas, Gorham Justin M, Pettibone John M, Tona Alessandro, Johnston-Peck Aaron, Liu Jingyu, Nelson Bryant C, Hackley Vincent A
Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899, United States.
ACS Appl Nano Mater. 2024;7(1). doi: 10.1021/acsanm.3c04935.
Using dendron chemistry, we developed stability enhanced, carboxylate surface modified (negatively charged dendron) AuNPs (Au-NCD). Since the carboxylate surface of Au-NCD is optimal for complexation with cisplatin (Pt) moieties, we further synthesized Pt loaded Au-NCD (Au-NCD/Pt) to serve as potential therapeutic anticancer agents. The size distribution, zeta potential and surface plasmon resonance of both Au-NCDs and Au-NCD/Pt were characterized via dynamic light scattering, scanning transmission electron microscopy and ultraviolet-visible spectrophotometry. Surface chemistry, Pt uptake, and Pt release were evaluated using inductively coupled plasma-mass spectrometry and X-ray photoelectron spectroscopy. Colloidal stability in physiological media over a wide pH range (1 to 13) and shelf-life stability (up to 6 months) were also assessed. Finally, the cytotoxicity of both Au-NCD and Au-NCD/Pt to Chinese hamster ovary cells (CHO K1; as a normal cell line) and to human lung epithelial cells (A549; as a cancer cell line) were evaluated. The results of these physicochemical and functional cytotoxicity studies with Au-NCD/Pt demonstrated that the particles exhibited superlative colloidal stability, cisplatin uptake and in vitro anticancer activity despite low amounts of Pt release from the conjugate.
利用树枝状化学，我们开发了稳定性增强、羧酸盐表面修饰（带负电荷的树枝状分子）的金纳米颗粒（Au-NCD）。由于Au-NCD的羧酸盐表面对于与顺铂（Pt）部分络合而言是最佳的，我们进一步合成了负载Pt的Au-NCD（Au-NCD/Pt），以用作潜在的治疗性抗癌剂。通过动态光散射、扫描透射电子显微镜和紫外可见分光光度法对Au-NCD和Au-NCD/Pt的尺寸分布、zeta电位和表面等离子体共振进行了表征。使用电感耦合等离子体质谱和X射线光电子能谱评估了表面化学、Pt摄取和Pt释放。还评估了在较宽pH范围（1至13）的生理介质中的胶体稳定性和保质期稳定性（长达6个月）。最后，评估了Au-NCD和Au-NCD/Pt对中国仓鼠卵巢细胞（CHO K1；作为正常细胞系）和人肺上皮细胞（A549；作为癌细胞系）的细胞毒性。这些对Au-NCD/Pt进行的物理化学和功能细胞毒性研究结果表明，尽管结合物中Pt释放量较低，但这些颗粒仍表现出极佳的胶体稳定性、顺铂摄取和体外抗癌活性。
