Howie A J, Bliss D J, Brewer D B
J Pathol. 1985 Mar;145(3):213-27. doi: 10.1002/path.1711450303.
Female Munich-Wistar rats were given intraperitoneal injections either of bovine serum albumin to induce proteinuria or of water as a control. Their kidneys were fixed in situ. An ultrastructural technique was used to demonstrate IgG antiperoxidase antibodies either injected from a heterologous species or autologous, induced by immunization with horseradish peroxidase. Photometry of electron micrographic negative was used to determine the distribution of antiperoxidase antibodies. In glomeruli of control animals IgG was present in the basement membrane. There were three sites at which the passage of IgG across the basement membrane was hindered: between blood plasma and the lamina rara interna, between the lamina densa and the lamina rara externa, and between the lamina rara externa and the urinary space. Glomeruli of proteinuric animals were variable in appearance, some showing little structural damage and others showing marked changes with loss of epithelial foot processes and accumulation of vacuoles and protein droplets in epithelial cells. Both types of glomeruli contained IgG in the urinary space. The distribution of IgG in the basement membrane of both types was similar. Compared with control animals there was less IgG in the basement membrane and IgG was distributed uniformly across the basement membrane. The proteinuria in hyperalbuminaemia (protein-overload) is associated with a diffuse change in the barrier function of the glomerular basement membrane to IgG which is, at least in the initial stages, not related to structural changes in glomerular epithelial cells.
将雌性慕尼黑 - 威斯塔大鼠分为两组，一组腹腔注射牛血清白蛋白以诱导蛋白尿，另一组注射水作为对照。将它们的肾脏原位固定。采用超微结构技术来显示从异种注射或自体注射的IgG抗过氧化物酶抗体，后者是通过用辣根过氧化物酶免疫诱导产生的。利用电子显微镜底片的光度测定法来确定抗过氧化物酶抗体的分布。在对照动物的肾小球中，IgG存在于基底膜中。IgG穿过基底膜的过程在三个部位受到阻碍：血浆与内疏松层之间、致密层与外疏松层之间以及外疏松层与尿腔之间。蛋白尿动物的肾小球外观各异，一些几乎没有结构损伤，而另一些则有明显变化，表现为上皮足突消失，上皮细胞内出现空泡和蛋白滴积聚。两种类型的肾小球在尿腔中均含有IgG。两种类型肾小球基底膜中IgG的分布相似。与对照动物相比，基底膜中的IgG较少，且IgG在基底膜上均匀分布。高白蛋白血症（蛋白过载）中的蛋白尿与肾小球基底膜对IgG的屏障功能的弥漫性改变有关，至少在初始阶段，这与肾小球上皮细胞的结构变化无关。
