Olivetti G, Kithier K, Giacomelli F, Wiener J
Lab Invest. 1981 Feb;44(2):127-37.
The distribution of endogenous albumin and immunoglobulin G (IgG) has been studied by the immunoperoxidase technique in the superficial glomeruli of both normotensive and hypertensive (acute angiotensin II-induced) Munich-Wistar rats. Endogenous IgG has also been detected in rats immunized with horseradish peroxidase. Labeled antibodies have been applied to sections on a conventional manner as well as by an electrophoretic technique. The immunization of animals with horseradish peroxidase, as well as application of the electrophoretic technique, both result in a greater yield of labeled glomeruli. Albumin is present within the capillary lumina of control animals, penetrates the capillary walls, and extends into the urinary space. Endogenous IgG is mainly confined to lumina of glomerular capillaries, with only small amounts visible in the laminae rarae of the basement membrane. After acute hypertension induced by angiotensin II, there is increased staining of albumin and IgG in the basement membrane and of albumin in the urinary space. There is also penetration of IgG into Bowman's space. Both macromolecules are found in dilated mesangial channels. These modifications of glomerular permselectivity in hypertension are not accompanied by discernible ultrastructural changes in the peripheral capillary wall. It is suggested that the transcapillary passage of albumin and IgG is dependent upon hemodynamic factors and/or subtle changes in the filtering membrane.
采用免疫过氧化物酶技术，对正常血压和高血压（急性血管紧张素II诱导）的慕尼黑-威斯塔大鼠浅表肾小球内源性白蛋白和免疫球蛋白G（IgG）的分布进行了研究。在用辣根过氧化物酶免疫的大鼠中也检测到了内源性IgG。标记抗体已通过传统方式以及电泳技术应用于切片。用辣根过氧化物酶免疫动物以及应用电泳技术，均能使标记肾小球的产量更高。白蛋白存在于对照动物的毛细血管腔内，穿过毛细血管壁，并延伸至尿腔。内源性IgG主要局限于肾小球毛细血管腔，仅在基底膜的透明层中可见少量。血管紧张素II诱导急性高血压后，基底膜中白蛋白和IgG以及尿腔中白蛋白的染色增加。IgG也渗入鲍曼腔。在扩张的系膜通道中发现了这两种大分子物质。高血压时肾小球滤过选择性的这些改变，并未伴有外周毛细血管壁明显的超微结构变化。提示白蛋白和IgG的跨毛细血管转运依赖于血流动力学因素和/或滤过膜的细微变化。
