Department of Medical Laboratory Science, Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, China.
Department of Clinical Laboratory, Brain Hospital of Hunan Province the Second People's Hospital of Hunan Province, Changsha, Hunan, China.
Medicine (Baltimore). 2024 Jun 7;103(23):e38416. doi: 10.1097/MD.0000000000038416.
To investigate the expression of Inhibin B between various clinical stages, Chinese medicine dialectic typing, and in nasopharyngeal carcinoma (NPC) tissues and serum, and to evaluate the potential of Inhibin B as a new biomarker for NPC. Paraffin specimens of pathologically confirmed NPC tissues and paracancerous tissues were retrospectively collected, and the expression of Inhibin α (INHA) and Inhibin βB (INHBB) was detected by SP method, and their relationship with clinicopathological indexes was analyzed; in addition, patients with NPC who had received radiotherapy were included as the study subjects, and Epstein-Barr virus DNA (EBV-DNA), INHA, and INHBB in patients were detected by using the fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and chemiluminescent immuno-sandwiching method, respectively. EBV-DNA, EBV-viral capsid antigen-immunoglobulin A (VCA IgA), INHA, and INHBB were detected in the patients, respectively, and their relationships with traditional Chinese medicine (TCM) patterns were also analyzed. The expression of INHA and INHBB in NPC tissues was lower than that in paracancerous tissues, and the expression of INHA in NPC patients was correlated with lymphatic metastasis, clinical staging, and TCM staging; the levels of EBV-DNA and VCA IgA were higher than that of healthy populations in NPC patients and were higher than that of patients with stage III + IV than that of patients with stage I + II, and the levels of INHA and INHBB were lower than those of healthy populations and were lower than those of patients with stage III + IV than that of patients with stage I + II. The levels of INHA and INHBB in nasopharyngeal cancer patients were lower than those in healthy people, and the levels in stage III + IV patients were lower than those in stage I + II patients. The levels of EBV-DNA and VCA IgA in nasopharyngeal cancer patients were correlated with the Chinese medicine patterns, and had different patterns. The expression of Inhibin B may be related to the progression of NPC, and it has certain typing significance for different TCM syndromes of NPC, which is helpful for TCM typing diagnosis.
为了研究抑制素 B 在不同临床阶段、中医辨证分型以及鼻咽癌（NPC）组织和血清中的表达，并评估其作为 NPC 新生物标志物的潜力。回顾性收集经病理证实的 NPC 组织和癌旁组织的石蜡标本，采用 SP 法检测抑制素 α（INHA）和抑制素 βB（INHBB）的表达，并分析其与临床病理指标的关系；此外，还纳入接受放疗的 NPC 患者作为研究对象，采用荧光定量聚合酶链反应、酶联免疫吸附试验和化学发光免疫夹心法分别检测患者的 EBV-DNA、INHA 和 INHBB。分别检测 NPC 患者的 EBV-DNA、EBV 衣壳抗原免疫球蛋白 A（VCA IgA）、INHA 和 INHBB，并分析其与中医（TCM）证型的关系。NPC 组织中 INHA 和 INHBB 的表达低于癌旁组织，NPC 患者中 INHA 的表达与淋巴转移、临床分期和 TCM 分期相关；NPC 患者的 EBV-DNA 和 VCA IgA 水平高于健康人群，且 III+IV 期患者高于 I+II 期患者，而 INHA 和 INHBB 水平低于健康人群，且 III+IV 期患者低于 I+II 期患者。鼻咽癌患者的 INHA 和 INHBB 水平低于健康人群，III+IV 期患者低于 I+II 期患者。鼻咽癌患者的 EBV-DNA 和 VCA IgA 水平与中医证型相关，且具有不同的证型。抑制素 B 的表达可能与 NPC 的进展有关，对 NPC 不同的 TCM 证型具有一定的分型意义，有助于 TCM 辨证诊断。
