缺氧诱导因子 1α 和 CD147 在三阴性乳腺癌发展中的潜在机制。
The potential mechanism of HIF-1α and CD147 in the development of triple-negative breast cancer.
机构信息
School of Medicine, Wuhan University of Science and Technology, Wuhan, People's Republic of China.
Department of Breast and Thyroid Surgery, Xiaogan Central Hospital and Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, People's Republic of China.
出版信息
Medicine (Baltimore). 2024 Jun 7;103(23):e38434. doi: 10.1097/MD.0000000000038434.
BACKGROUND
Triple-negative breast cancer (TNBC) is a subtype of breast cancer with a poor prognosis, and the outcomes of common therapy were not favorable.
METHODS
The samples of 84 patients with TNBC and 40 patients with breast fibroadenoma were collected in the pathology department specimen library of our hospital. The prognosis of patients was obtained through outpatient follow-up information, telephone and WeChat contacts, and medical records. The mRNA expression was analyzed using bioinformation and quantitative real-time polymerase chain reaction (qPCR). The protein expression was determined by hematoxylin-eosin staining and immunohistochemical staining. The results of survival analysis were visualized using Kaplan-Meier curves.
RESULTS
The immunohistochemical staining showed that hypoxia-inducible factor-1alpha (HIF-1α) was mainly distributed in the nucleus and cytoplasm, while CD147 is mainly distributed in cell membrane and cytoplasm. The qPCR results exhibited that the expression level of HIF-1α and CD147 in TNBC tissue was significantly higher than that in breast fibroadenoma tissue. The expression of HIF-1α was related to the histological grade and lymph node metastasis in TNBC, and the expression of CD147 was related to Ki-67, histological grade and lymph node metastasis. There was a positive relationship between the expression of CD147 and HIF-1α. The upregulated expression of CD147 was closely related to the poor prognosis of OS in TNBC.
CONCLUSION
CD147 could be a biomarker for the prognosis of TNBC and closely related to the expression of HIF-1α.
背景
三阴性乳腺癌（TNBC）是一种预后较差的乳腺癌亚型，常规治疗效果并不理想。
方法
从我院病理科标本库收集了 84 例 TNBC 患者和 40 例乳腺纤维腺瘤患者的样本。通过门诊随访信息、电话和微信联系以及病历获得患者的预后情况。使用生物信息学和实时定量聚合酶链反应（qPCR）分析 mRNA 表达。通过苏木精-伊红染色和免疫组织化学染色测定蛋白表达。使用 Kaplan-Meier 曲线可视化生存分析结果。
结果
免疫组织化学染色显示缺氧诱导因子-1α（HIF-1α）主要分布在细胞核和细胞质中，而 CD147 主要分布在细胞膜和细胞质中。qPCR 结果显示，TNBC 组织中 HIF-1α 和 CD147 的表达水平明显高于乳腺纤维腺瘤组织。HIF-1α 的表达与 TNBC 的组织学分级和淋巴结转移有关，CD147 的表达与 Ki-67、组织学分级和淋巴结转移有关。CD147 的表达与 HIF-1α 呈正相关。CD147 的上调表达与 TNBC 的 OS 预后不良密切相关。
结论
CD147 可能是 TNBC 预后的生物标志物，与 HIF-1α 的表达密切相关。
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