State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China; Chongqing Research Institute of HIT, Chongqing, 401151, China.
Environ Pollut. 2024 Sep 1;356:124331. doi: 10.1016/j.envpol.2024.124331. Epub 2024 Jun 5.
The presence of both chlorine-resistant bacteria (CRB) and microplastics (MPs) in drinking water distribution systems (DWDS) poses a threat to water quality and human health. However, the risk of CRB bio evolution under the stress of MPs remains unclear. In this study, polypropylene (PP) and polyethylene (PE) were selected to study the adsorption and desorption behavior of sulfamethoxazole (SMX), and it was clear that MPs had the risk of carrying pollutants into DWDS and releasing them. The results of the antibiotic susceptibility test and disinfection experiment confirmed that MPs could enhance the resistance of CRB to antibiotics and disinfectants. Bacteria epigenetic resistance mechanisms were approached from multiple perspectives, including physiological and biochemical characteristics, as well as molecular regulatory networks. When MPs enter DWDS, CRB could attach to the surface of MPs and directly interact with both MPs and the antibiotics they release. This attachment process promoted changes in the composition and content of extracellular polymers (EPS) within cells, enhanced surface hydrophobicity, stimulated oxidative stress function, and notably elevated the relative abundance of certain antibiotic resistance genes (ARGs). This study elucidates the mechanism by which MPs alter the intrinsic properties of CRB, providing valuable insights into the effective avoidance of biological risks to water quality during CRB evolution.
饮用水分配系统（DWDS）中同时存在抗氯细菌（CRB）和微塑料（MPs）会对水质和人类健康构成威胁。然而，在 MPs 压力下 CRB 生物进化的风险尚不清楚。在这项研究中，选择了聚丙烯（PP）和聚乙烯（PE）来研究磺胺甲恶唑（SMX）的吸附和解吸行为，很明显 MPs 有将污染物带入 DWDS 并释放它们的风险。抗生素敏感性测试和消毒实验的结果证实， MPs 可以增强 CRB 对抗生素和消毒剂的耐药性。从生理生化特性和分子调控网络等多个角度探讨了细菌表观遗传耐药机制。当 MPs 进入 DWDS 时，CRB 可以附着在 MPs 的表面上，并直接与 MPs 及其释放的抗生素相互作用。这种附着过程促进了细胞内细胞外聚合物（EPS）组成和含量的变化，增强了表面疏水性，刺激了氧化应激功能，并显著提高了某些抗生素耐药基因（ARGs）的相对丰度。本研究阐明了 MPs 改变 CRB 内在特性的机制，为有效避免 CRB 进化过程中水质的生物风险提供了有价值的见解。
