转录组分析揭示了不同发育阶段绵羊肌肉组织中基因表达的差异。

Transcriptome analysis revealed differences in gene expression in sheep muscle tissue at different developmental stages.

机构信息

College of Agricultural Engineering, Anhui Open University, Hefei, 230022, P.R. China.

College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036, P.R. China.

出版信息

BMC Genom Data. 2024 Jun 7;25(1):54. doi: 10.1186/s12863-024-01235-9.

DOI:10.1186/s12863-024-01235-9

PMID:38849746

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11162047/

Abstract

BACKGROUND

The analysis of differentially expressed genes in muscle tissues of sheep at different ages is helpful to analyze the gene expression trends during muscle development. In this study, the longissimus dorsi muscle of pure breeding Hu sheep (H), Suffolk sheep and Hu sheep hybrid F1 generation (SH) and East Friesian and Hu sheep hybrid sheep (EHH) three strains of sheep born 2 days (B2) and 8 months (M8) was used as the research object, and transcriptome sequencing technology was used to identify the differentially expressed genes of sheep longissimus dorsi muscle in these two stages. Subsequently, GO and KEGG enrichment analysis were performed on the differential genes. Nine differentially expressed genes were randomly selected and their expression levels were verified by qRT-PCR.

RESULTS

The results showed that 842, 1301 and 1137 differentially expressed genes were identified in H group, SH group and EHH group, respectively. Among them, 191 differential genes were enriched in these three strains, including pre-folding protein subunit 6 (PFDN6), DnaJ heat shock protein family member A4 (DNAJA4), myosin heavy chain 8 (MYH8) and so on. GO and KEGG enrichment analysis was performed on 191 differentially expressed genes shared by the three strains to determine common biological pathways. The results showed that the differentially expressed genes were significantly enriched in ribosomes, unfolded protein binding, FoxO signaling pathway, glycolysis / glycogen generation and glutathione signaling pathway that regulate muscle protein synthesis and energy metabolism. The results of qRT-PCR were consistent with transcriptome sequencing, which proved that the sequencing results were reliable.

CONCLUSIONS

Overall, this study revealed the important genes and signaling pathways related to sheep skeletal muscle development, and the result laid a foundation for further understanding the mechanism of sheep skeletal muscle development.

摘要

背景

分析不同年龄绵羊肌肉组织中的差异表达基因有助于分析肌肉发育过程中的基因表达趋势。本研究以纯种湖羊（H）、萨福克羊和湖羊杂种 F1 代（SH）以及东弗里生羊和湖羊杂种羊（EHH）3 个品种绵羊出生 2 天（B2）和 8 个月（M8）时的背最长肌为研究对象，采用转录组测序技术鉴定这两个阶段绵羊背最长肌的差异表达基因。随后对差异基因进行 GO 和 KEGG 富集分析。随机选择 9 个差异表达基因，通过 qRT-PCR 验证其表达水平。

结果

结果表明，H 组、SH 组和 EHH 组分别鉴定出 842、1301 和 1137 个差异表达基因。其中，这 3 个品系共有 191 个差异基因富集，包括前折叠蛋白亚基 6（PFDN6）、DNAJ 热休克蛋白家族成员 A4（DNAJA4）、肌球蛋白重链 8（MYH8）等。对 3 个品系共有的 191 个差异表达基因进行 GO 和 KEGG 富集分析，确定共同的生物学途径。结果表明，差异表达基因在核糖体、未折叠蛋白结合、FoxO 信号通路、糖酵解/糖原生成和谷胱甘肽信号通路中显著富集，这些途径调节肌肉蛋白合成和能量代谢。qRT-PCR 结果与转录组测序结果一致，证明测序结果可靠。