Zhao Zhonglong, Zhang Yong, Yang Hong, Wang Zhiwei, Ai Zhaobi, Yang Runqian, Wang Tiansong, Ye Li, Shu Chang
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang, 550025, China.
Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science, Guizhou University, Guiyang, 550025, China.
Trop Anim Health Prod. 2025 Jun 17;57(6):274. doi: 10.1007/s11250-025-04524-2.
Adipose tissue deposition patterns in poultry significantly influence meat quality and production efficiency. However, the molecular mechanisms regulating differential fat accumulation between adipose and muscle tissues remain poorly understood in waterfowl. To evaluate and compare the underlying molecular processes of fat accumulation in Zhijin white geese abdominal fat (AF) and breast muscle (BM). The differential gene expression profiles in the AF and BM tissues were conducted by using RNA-seq. Six male Zhijin white geese, all 180 days old and of equivalent body weight, were selected for collecting AF and BM tissue samples. After RNA-seq, Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway enrichment analysis was done to identify differentially expressed genes (DEGs) associated with adipose tissue deposition and lipid metabolism. Randomly selected 7 DEGs for real-time fluorescence quantitative PCR (qRT-PCR) validation in order to confirm the accuracy of the sequencing data. The results showed that there were 5969 DEGs in the AF and BM tissues of Zhijin white geese, of which 2860 were upregulated and 3109 were downregulated. The Gene Ontology (GO) functional annotation analysis demonstrated that DEGs were primarily implicated in lipid localization, phosphorus metabolic processes, catalytic activity, and other pathways associated with lipid metabolism. The citrate cycle (TCA cycle), pentose phosphate pathway, and glycolysis/gluconeogenesis were identified as KEGG pathways with significant enrichment. Transcriptome profiling and bioinformatic analysis of the AF and BM tissues revealed that the genes ALDOA, PDHA1, PGM1, ALDOC, PFKL, PDHX, PRPS1, SDHA and GAPDH were involved in lipid metabolism. These genes may play a crucial role in controlling fat deposition in AF and BM tissues of Zhijin white goose.
家禽体内脂肪组织的沉积模式对肉质和生产效率有显著影响。然而，水禽体内脂肪组织和肌肉组织之间差异脂肪积累的调控分子机制仍知之甚少。为了评估和比较织金白鹅腹部脂肪（AF）和胸肌（BM）中脂肪积累的潜在分子过程。通过RNA测序对AF和BM组织中的差异基因表达谱进行了分析。选取6只180日龄、体重相当的雄性织金白鹅，采集AF和BM组织样本。RNA测序后，进行京都基因与基因组百科全书（KEGG）信号通路富集分析，以鉴定与脂肪组织沉积和脂质代谢相关的差异表达基因（DEG）。随机选择7个DEG进行实时荧光定量PCR（qRT-PCR）验证，以确认测序数据的准确性。结果表明，织金白鹅AF和BM组织中有5969个DEG，其中2860个上调，3109个下调。基因本体论（GO）功能注释分析表明，DEG主要参与脂质定位、磷代谢过程、催化活性以及其他与脂质代谢相关的途径。柠檬酸循环（TCA循环）、磷酸戊糖途径和糖酵解/糖异生被确定为KEGG中显著富集的途径。AF和BM组织的转录组分析和生物信息学分析表明，基因ALDOA、PDHA1、PGM1、ALDOC、PFKL、PDHX、PRPS1、SDHA和GAPDH参与脂质代谢。这些基因可能在控制织金白鹅AF和BM组织中的脂肪沉积方面发挥关键作用。
