Department of Restorative Dentistry, Bahcesehir University School of Dental Medicine, Istanbul, Türkiye.
School of Medicine and Health Science, Department of Therapeutic Dentistry, BAU International University, Batumi, Georgia.
BMC Oral Health. 2024 Jun 7;24(1):663. doi: 10.1186/s12903-024-04438-9.
Restorative materials are in prolonged contact with living tissues such as oral mucosa, dentin, pulp, periodontal, and periapical tissues. Therefore, the potentially harmful effects of these materials and their components on oral tissues should be evaluated before clinical use. This study aimed to compare the cell viability of different adhesive systems (ASs) on human dental pulp stem cells (hDPSCs).
Three ASs that combining methacryloyloxydecyl dihydrogen phosphate (MDP) monomer with new hydrophilic amide monomers [Clearfil Universal Bond Quick(CUBQ), Kuraray Noritake], self-reinforcing 3D monomer [Bond Force II(BFII), Tokuyama)], and dual-cure property [Futurabond DC(FBDC), VOCO] were used. Three (n = 3) samples were prepared for each group. Dental pulp stem cells were isolated from ten patients' extracted third molar teeth. Samples were incubated in Dulbecco's modified Eagle's medium (DMEM) for 24 h (h), 72 h, and 7 days (d) to obtain extracts. For the control group, cells were cultured without DBA samples. Cell viability of ASs extracts was measured using a cell proliferation detection kit (WST-1, Roche). Statistical analysis was performed using two-way ANOVA and post-hoc (Duncan) tests (p < 0.05).
At 24 and 72 h statistically significant differences were determined between control and BFII, control and FBDC groups (p < 0.05), while no differences between control and CUBQ groups (p > 0.05). On the 7th d, statistically significant differences were found between the control and experimental groups (p < 0.05), while no differences between experimental groups (p > 0.05). A statistically significant difference was detected for the BFII group over the three-time interval (p < 0.05). The lowest cell viability was observed for the FBDC group at 24 h, and the difference was statistically significant when compared with 72 h and 7th d (p < 0.05).
All ASs showed different cell viability values at various exposure times. It should be taken into consideration that pH values, as well as the contents of ASs, have a significant effect on the cell viability.
修复材料与口腔黏膜、牙本质、牙髓、牙周和根尖组织等活组织长时间接触。因此，在临床使用前，应评估这些材料及其成分对口腔组织的潜在有害影响。本研究旨在比较不同粘接系统（AS）对人牙髓干细胞（hDPSCs）的细胞活力。
使用三种结合了 MDP 单体和新型亲水性酰胺单体的 AS [Clearfil Universal Bond Quick(CUBQ)、Kuraray Noritake]、自增强 3D 单体 [Bond Force II(BFII)、Tokuyama)]和双固化性能 [Futurabond DC(FBDC)、VOCO]。每组各制备三个(n = 3)样本。从 10 名患者的第三磨牙中分离牙髓干细胞。将样本在 Dulbecco 改良 Eagle 培养基 (DMEM) 中孵育 24 小时（h）、72 小时（h）和 7 天（d）以获得提取物。对于对照组，细胞在没有 DBA 样本的情况下培养。使用细胞增殖检测试剂盒 (WST-1, Roche) 测量 AS 提取物的细胞活力。使用双因素方差分析和事后 (Duncan) 检验进行统计分析（p < 0.05）。
在 24 小时和 72 小时，对照组与 BFII 组、对照组与 FBDC 组之间存在统计学显著差异（p < 0.05），而对照组与 CUBQ 组之间无差异（p > 0.05）。在第 7 天，对照组与实验组之间存在统计学显著差异（p < 0.05），而实验组之间无差异（p > 0.05）。BFII 组在三个时间间隔内均存在统计学显著差异（p < 0.05）。FBDC 组在 24 小时时细胞活力最低，与 72 小时和第 7 天相比差异具有统计学意义（p < 0.05）。
所有 AS 在不同暴露时间均显示出不同的细胞活力值。应考虑到 pH 值以及 AS 的含量对细胞活力有显著影响。
