Demırbuga Sezer, Pala Kanşad, Topçuoğlu Hüseyin Sinan, Çayabatmaz Muhammed, Topçuoğlu Gamze, Uçar Ebru Nur
Department of Restorative Dentistry, Faculty of Dentistry, Erciyes University, Kayseri, Turkey.
Department of Endodontics, Faculty of Dentistry, Erciyes University, Melikgazi, 38039, Kayseri, Turkey.
Clin Oral Investig. 2017 Mar;21(2):627-633. doi: 10.1007/s00784-016-1929-6. Epub 2016 Aug 2.
The aim of this study was to evaluate the effect of different endodontic solvents on the microtensile bond strength (μTBS) of various adhesives to pulp chamber dentin.
A total of 120 human third molars were selected. Canals were prepared with the ProTaper Universal system and obturated. The access cavities were then restored with resin composite. After 1 week, a retreatment procedure was applied as follows: control, no solvent was applied to the pulp chamber and experimental groups, three different solvents (chloroform, eucalyptol, and orange oil) were applied to the pulp chamber for 2 min. The canal filling was removed and calcium hydroxide (Ca[OH]) was placed into the canals. After 7 days, the Ca(OH) was removed from the canals and the canals were re-obturated. Teeth were then divided into three subgroups according to the adhesive used. The samples were restored with a nanohybrid resin composite using three different adhesives: Clearfil SE Bond (CSE), Adper Easy One (AEO), and Single Bond 2 (SB2). The samples were aged with thermocycling. Teeth were sectioned, and a total of 20 dentin sticks were obtained for each subgroup. μTBS testing was then performed. The debonded surfaces were evaluated using scanning electron microscopy (SEM) analysis. Data were analyzed using two-way ANOVA and Tukey's post hoc tests.
Chloroform showed statistically lower mean μTBS values (14 ± 7.2 MPa) than control group did (19.2 ± 6.1 MPa) (p < 0.05). Orange oil (18.1 ± 6.3 MPa) and eucalyptol (16.9 ± 6.8 MPa) did not reduce the mean μTBS statistically (p > 0.05). Chloroform showed significantly lower bond strength for all adhesives (p < 0.05). Whereas orange oil did not reduce the mean μTBS values of all adhesive systems significantly (p > 0.05), eucalyptol reduced the μTBS values of all the groups, but the results were only statistically significant for SB2 (p < 0.05). CSE showed statistically higher bond strength (20.4 ± 6.8 MPa) than AEO (14.6 ± 5.3 MPa) and SB2 (16.3 ± 7.2 MPa) did (p < 0.05). There were no statistical differences between AEO and SB2 (p > 0.05). According to the SEM analysis of the debonded surfaces, adhesive failures were the most common type in all the groups, followed by mixed failures.
While chloroform reduced the mean bond strength of the adhesive resins, orange oil did not affect the bond strength of the adhesives. The effect of eucalyptol on bond strength depended on the type of adhesive system.
This study shows that endodontic solvents could affect the microtensile bond strength of adhesives to pulp chamber dentin.
本研究旨在评估不同的根管内溶剂对各种粘结剂与髓腔牙本质之间微拉伸粘结强度（μTBS）的影响。
共选取120颗人第三磨牙。使用ProTaper Universal系统预备根管并进行充填。然后用树脂复合材料修复髓腔。1周后，进行如下再治疗程序：对照组，不向髓腔施加任何溶剂；实验组，将三种不同的溶剂（氯仿、桉叶油和橙油）施加于髓腔2分钟。去除根管充填物，向根管内放置氢氧化钙（Ca[OH]）。7天后，从根管内取出Ca(OH)，重新充填根管。然后根据所使用的粘结剂将牙齿分为三个亚组。使用三种不同的粘结剂（Clearfil SE Bond（CSE）、Adper Easy One（AEO）和Single Bond 2（SB2））用纳米混合树脂复合材料修复样本。对样本进行热循环老化处理。将牙齿切片，每个亚组共获得20根牙本质棒。然后进行μTBS测试。使用扫描电子显微镜（SEM）分析脱粘表面。数据采用双向方差分析和Tukey事后检验进行分析。
氯仿的平均μTBS值（14±7.2MPa）在统计学上低于对照组（19.2±6.1MPa）（p<0.05）。橙油（18.1±6.3MPa）和桉叶油（16.9±6.8MPa）在统计学上未降低平均μTBS值（p>0.05）。氯仿对所有粘结剂的粘结强度均有显著降低（p<0.05）。橙油未显著降低所有粘结剂系统的平均μTBS值（p>0.05），而桉叶油降低了所有组的μTBS值，但仅对SB2组具有统计学意义（p<0.05）。CSE的粘结强度（20.4±6.8MPa）在统计学上高于AEO（14.6±5.3MPa）和SB2（16.3±7.2MPa）（p<0.05）。AEO和SB2之间无统计学差异（p>0.05）。根据脱粘表面的SEM分析，粘结剂断裂是所有组中最常见的类型，其次是混合断裂。
氯仿降低了粘结树脂的平均粘结强度，而橙油未影响粘结剂的粘结强度。桉叶油对粘结强度的影响取决于粘结剂系统的类型。
本研究表明根管内溶剂可能会影响粘结剂与髓腔牙本质之间的微拉伸粘结强度。
