State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510641, China.
Int J Biol Macromol. 2024 Jul;273(Pt 2):133003. doi: 10.1016/j.ijbiomac.2024.133003. Epub 2024 Jun 6.
Enzymatic hydrolysis is important for lignocellulosic biomass conversion into fermentable sugars. However, the nonproductive adsorption of enzyme on lignin was major hinderance for the enzymatic hydrolysis efficiency. In this study, non-productive adsorption mechanism of cellulase component cellobiohydrolase (CBH) onto lignin was specific investigated. Research revealed that the adsorption behavior of CBH on eucalyptus alkali lignin (E) was affected by reaction conditions. As study on the adsorption kinetic, it was indicated that the adsorption cellulose binding domain (CBD) of CBH onto E well fitted with Langmuir adsorption model and pseudo second-order adsorption kinetics model. And the tyrosine site related to the adsorption of CBD onto lignin was proved by the fluorescence and UV spectra analysis. The results of this work provide a theoretical guidance to understanding the nonproductive adsorption mechanism and building method to reduce the adsorption of cellulase on the lignin.
酶解对于将木质纤维素生物质转化为可发酵糖非常重要。然而，酶在木质素上的非生产性吸附是影响酶解效率的主要障碍。在这项研究中，我们专门研究了纤维素酶组分纤维二糖水解酶（CBH）在木质素上的非生产性吸附机制。研究表明，CBH 在桉木堿木质素（E）上的吸附行为受反应条件的影响。通过对吸附动力学的研究，表明 CBH 的纤维素结合域（CBD）在 E 上的吸附很好地符合朗缪尔吸附模型和准二级吸附动力学模型。并且通过荧光和紫外光谱分析证明了与 CBD 吸附到木质素相关的酪氨酸位点。这项工作的结果为理解非生产性吸附机制提供了理论指导，并为减少纤维素酶在木质素上的吸附提供了方法。
