Division of Tropical and Infection Disease, Department of Internal Medicine Dr. Moewardi General Hospital/Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126 Indonesia.
Department of Internal Medicine Dr. Moewardi General Hospital/Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126 Indonesia.
Trop Biomed. 2024 Mar 1;41(1):14-19. doi: 10.47665/tb.41.1.002.
Infectious diseases with complications of sepsis are still public health concern in both developed and developing countries. Sepsis is a potentially life-threatening systemic immune response to infection that can lead to end- stage organ failure and death. Sepsis involves multiple mechanisms such as neuroendocrine, complement activation, blood coagulation, and fibrinolytic system. Reactive oxygen species (ROS) is an inflammatory mediator produced by NADPH oxidase activation. This study aimed to investigate the effects of ethanol extract of E. elatior fruits on NADPH oxidase activity. Forty Mus musculus mice were randomized divided into five groups (n=8), with the intervention group receiving an intraperitoneal injection of 0.3 mg/kg BW lipopolysaccharide (LPS). There was a normal group without LPS injection (N-1), LPS injection only (N-2), and those that received LPS injection and ethanol extracts of E. elatior fruits containing 2.1 mg/20 g (N-3), 4.2 mg/20 g (N-4), and 8.4 mg/20 g (N-5). NADPH oxidase activity were measured using ELISA. The oneway ANOVA was used to investigate the differences between the groups. After administration of the extract at a varied dose, N-5 group the lowest NADPH oxidase activity (p=0.001). The ethanol extract of E. elatior fruit has antioxidant effects. In this study, a dose of 8.4 mg/20 g of extract significantly reduced NADPH oxidase activity. The ethanol extract of E. elatior might be considered a treatment in sepsis.
感染性疾病并发脓毒症仍然是发达国家和发展中国家共同面临的公共卫生问题。脓毒症是一种潜在的危及生命的全身炎症反应综合征，可导致终末期器官衰竭和死亡。脓毒症涉及多个机制，如神经内分泌、补体激活、凝血和纤维蛋白溶解系统。活性氧（ROS）是一种由 NADPH 氧化酶激活产生的炎症介质。本研究旨在探讨绿刺柏果实乙醇提取物对 NADPH 氧化酶活性的影响。将 40 只 Mus musculus 小鼠随机分为五组（n=8），干预组给予腹腔注射 0.3mg/kg BW 脂多糖（LPS）。设无 LPS 注射的正常组（N-1）、仅 LPS 注射组（N-2）、给予 LPS 注射和绿刺柏果实乙醇提取物 2.1mg/20g（N-3）、4.2mg/20g（N-4）、8.4mg/20g（N-5）的组。采用 ELISA 法测定 NADPH 氧化酶活性。采用单因素方差分析比较组间差异。在给予不同剂量的提取物后，N-5 组 NADPH 氧化酶活性最低（p=0.001）。绿刺柏果实乙醇提取物具有抗氧化作用。在这项研究中，8.4mg/20g 剂量的提取物显著降低了 NADPH 氧化酶活性。绿刺柏果实乙醇提取物可能被认为是脓毒症的一种治疗方法。
