Laboratório de Bioquímica Metabólica (LBM), Departamento de Ciências Biológicas (DECBI), Programa de Pós-Graduação em Ciências Biológicas, Universidade Federal de Ouro Preto, Minas Gerais, Brazil.
Laboratório de Farmacognosia, Escola de Farmácia, Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
Oxid Med Cell Longev. 2018 Nov 7;2018:5048031. doi: 10.1155/2018/5048031. eCollection 2018.
Sepsis is a complex disease and is the cause of many deaths worldwide. Sepsis pathogenesis involves a dysregulated inflammatory response with consequent production of inflammatory mediators and reactive species. The production and excessive release of these substances into the systemic circulation trigger various cellular and metabolic alterations that are observed during the disease evolution. Thus, more studies have been carried out to investigate the therapeutic potential of plants such as L., popularly known as black mulberry. Studies have shown that plants belonging to the genus are rich in secondary metabolites such as flavonoids which are associated with important biological activities as antioxidant and anti-inflammatory actions. Based on this context, the objective of our study was to evaluate the anti-inflammatory and antioxidant properties of L. in a sepsis model induced by LPS. Male C57BL/6 mice were distributed in four groups: control, sepsis, sepsis treated with leaf extract of mulberry, and sepsis treated with mulberry pulp. The animals were treated with 100 L of their respective treatments for twenty-one days. Sepsis was induced at the 21st day with lipopolysaccharide (LPS) by intraperitoneal injection. The animals were euthanized 24 hours after receiving the LPS injection. The data obtained were analyzed in GraphPad Prism 6.0 software. Our results showed that treatment with either extract significantly decreased the number of leukocytes in the bronchoalveolar lavage fluid and serum levels of TNF in septic animals. Regarding the redox status, the treatments significantly decreased the antioxidant activity of the enzyme glutathione peroxidase. Regarding metalloproteinase type 2, it was observed that the treatment with black mulberry pulp was able to significantly reduce the activity of this enzyme concerning the sepsis group. Finally, these results together promoted an increase in the animal's survival that received the black mulberry leaf or pulp extract.
脓毒症是一种复杂的疾病，是全球许多死亡的原因。脓毒症的发病机制涉及失调的炎症反应，随后产生炎症介质和活性物质。这些物质在全身循环中的产生和过度释放会触发在疾病演变过程中观察到的各种细胞和代谢改变。因此，已经进行了更多的研究来研究植物如 L.（俗称黑桑）的治疗潜力。研究表明，属于 属的植物富含黄酮类等次生代谢物，这些物质与抗氧化和抗炎作用等重要的生物学活性有关。基于此背景，我们的研究目的是评估 LPS 诱导的脓毒症模型中 L.的抗炎和抗氧化特性。雄性 C57BL/6 小鼠分为四组：对照组、脓毒症组、用桑叶提取物治疗的脓毒症组和用桑椹果肉治疗的脓毒症组。动物用 100 μL 各自的处理剂处理 21 天。在第 21 天，通过腹腔内注射脂多糖（LPS）诱导脓毒症。在接受 LPS 注射后 24 小时处死动物。使用 GraphPad Prism 6.0 软件分析获得的数据。我们的结果表明，两种提取物的治疗均可显著减少脓毒症动物支气管肺泡灌洗液中的白细胞数和血清中 TNF 的水平。关于氧化还原状态，治疗可显著降低谷胱甘肽过氧化物酶的抗氧化活性。关于基质金属蛋白酶 2，观察到用黑桑椹果肉治疗可显著降低与脓毒症组相比该酶的活性。最后，这些结果共同促进了接受黑桑树叶或果肉提取物的动物的存活率提高。
