SDU-ANU Joint Science College, Weihai, Shandong, 264209, China.
Marine College, Shandong University, Weihai, Shandong, 264209, China.
Environ Pollut. 2024 Sep 1;356:124334. doi: 10.1016/j.envpol.2024.124334. Epub 2024 Jun 7.
Microplastics/nanoplastics (MNPs) inevitably coexist with other pollutants in the natural environment, making it crucial to study the interactions between MNPs and other pollutants as well as their combined toxic effects. In this study, we investigated neurotoxicity in marine medaka (Oryzias melastigma) exposed to polystyrene micro/nanoplastics (PS-MNPs), triphenyltin (TPT), and PS-MNPs + TPT from physiological, behavioral, biochemical, and genetic perspectives. The results showed that marine medaka exposed to 200 ng/L TPT or 200 μg/L PS-NPs alone exhibited some degree of neurodevelopmental deficit, albeit with no significant behavioral abnormalities observed. However, in the PS-MP single exposure group, the average acceleration of short-term behavioral indices was significantly increased by 78.81%, indicating a highly stress-responsive locomotor pattern exhibited by marine medaka. After exposure to PS-MNPs + TPT, the swimming ability of marine medaka significantly decreased. In addition, PS-MNPs + TPT exposure disrupted normal neural excitability as well as activated detoxification processes in marine medaka larvae. Notably, changes in neural-related genes suggested that combined exposure to PS-MNPs and TPT significantly increased the neurotoxic effects observed with exposure to PS-MNPs or TPT alone. Furthermore, compared to the PS-MPs + TPT group, PS-NPs + TPT significantly inhibited swimming behavior and thus exacerbated the neurotoxicity. Interestingly, the neurotoxicity of PS-MPs was more pronounced than that of PS-NPs in the exposure group alone. However, the addition of TPT significantly enhanced the neurotoxicity of PS-NPs compared to PS-MPs + TPT. Overall, the study underscores the combined neurotoxic effects of MNPs and TPT, providing in-depth insights into the ecotoxicological implications of MNPs coexisting with pollutants and furnishing comprehensive data.
微塑料/纳米塑料（MNPs）不可避免地与自然环境中的其他污染物共存，因此研究 MNPs 与其他污染物之间的相互作用及其联合毒性效应至关重要。在这项研究中，我们从生理、行为、生化和遗传角度研究了海洋稻（Oryzias melastigma）暴露于聚苯乙烯微/纳米塑料（PS-MNPs）、三苯基锡（TPT）和 PS-MNPs+TPT 时的神经毒性。结果表明，海洋稻单独暴露于 200ng/L TPT 或 200μg/L PS-NPs 时，表现出一定程度的神经发育缺陷，尽管没有观察到明显的行为异常。然而，在 PS-MP 单一暴露组中，短期行为指标的平均加速度显著增加了 78.81%，表明海洋稻表现出高度应激反应的运动模式。暴露于 PS-MNPs+TPT 后，海洋稻的游泳能力明显下降。此外，PS-MNPs+TPT 暴露破坏了海洋稻幼虫的正常神经兴奋性和激活了解毒过程。值得注意的是，神经相关基因的变化表明，PS-MNPs 和 TPT 的联合暴露显著增加了与 PS-MNPs 或 TPT 单独暴露观察到的神经毒性效应。此外，与 PS-MPs+TPT 组相比，PS-NPs+TPT 显著抑制了游泳行为，从而加剧了神经毒性。有趣的是，在单独暴露组中，PS-MPs 的神经毒性比 PS-NPs 更明显。然而，与 PS-MPs+TPT 相比，TPT 的添加显著增强了 PS-NPs 的神经毒性。总的来说，该研究强调了 MNPs 和 TPT 的联合神经毒性效应，深入了解了 MNPs 与污染物共存的生态毒理学意义，并提供了全面的数据。
