Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Food and Environment, Dalian University of Technology, Panjin 124221, China.
The Swire Institute of Marine Science and School of Biological Sciences, The University of Hong Kong, Pokfulam, Hong Kong, China; State Key Laboratory in Marine Pollution, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China.
Mar Pollut Bull. 2017 Nov 30;124(2):847-855. doi: 10.1016/j.marpolbul.2017.02.030. Epub 2017 Feb 24.
Toxic effects of triphenyltin (TPT) to different life stages of the marine medaka Oryzias melastigma were investigated through a series of life-cycle based exposure experiments. In embryo stage, TPT exposure could elevate the heartbeat rate at Day 6-8 post-fertilization and increase the expression levels of five heart development related genes (i.e., ATPase, COX2, BMP4, GATA4 and NKX2.5). In larval stage, TPT shortened the body length at ≥10μg/L and suppressed the swimming activity of the fish larvae at Day 1 post-hatching at 50μg/L. In reproductive stage, TPT exposure resulted in a male-biased sex ratio (2μg/L) and reduced the gonadosomatic index (GSI) in females (≥ 0.1μg/L), which might in turn lead to a decline in their population fitness. The reproductive stage of O. melastigma was more sensitive to TPT than other stages, while the GSI of female medaka was the most sensitive endpoint.
采用一系列基于生活史的暴露实验，研究了三苯基锡（TPT）对海洋稻鱼（Oryzias melastigma）不同生活阶段的毒性效应。在胚胎期，TPT 暴露可在受精后第 6-8 天提高心跳率，并增加五个心脏发育相关基因（即 ATPase、COX2、BMP4、GATA4 和 NKX2.5）的表达水平。在幼鱼期，10μg/L 以上的 TPT 暴露会缩短幼鱼的体长，而在孵化后第 1 天，50μg/L 的 TPT 会抑制幼鱼的游泳活动。在繁殖期，TPT 暴露导致雄性偏性性别比（2μg/L），并降低雌性的性腺指数（GSI）（≥0.1μg/L），这可能导致其种群适应性下降。稻鱼的繁殖期比其他阶段对 TPT 更敏感，而雌鱼的 GSI 是最敏感的终点。
