3D 打印义齿基托聚合物的后处理:离心方法对表面特性、弯曲性能和细胞毒性的影响。
Post-processing of a 3D-printed denture base polymer: Impact of a centrifugation method on the surface characteristics, flexural properties, and cytotoxicity.
机构信息
Department of Orthodontics, Stomatological Hospital, School of Stomatology, Southern Medical University, South Jiangnan Road No. 366, Guangzhou 510280, China.
Center of Oral Implantology, Stomatological Hospital, Southern Medical University, South Jiangnan Road No. 366, Guangzhou 510280, China.
出版信息
J Dent. 2024 Aug;147:105102. doi: 10.1016/j.jdent.2024.105102. Epub 2024 Jun 8.
OBJECTIVES
To investigate the impact of a centrifugation method on the surface characteristics, flexural properties, and cytotoxicity of an additively manufactured denture base polymer.
METHODS
The tested specimens were prepared by digital light processing (DLP). A centrifugation method (CENT) was used to remove the residual uncured resin. In addition, the specimens were post-processed with different post-rinsing solutions: isopropanol (IPA), ethanol (EtOH), and tripropylene glycol monomethyl ether (TPM), respectively. A commercial heat-polymerized polymethyl methacrylate was used as a reference (REF). First, the values of surface topography, arithmetical mean height (Sa), and root mean square height (Sq) were measured. Next, flexural strength (FS) and modulus were evaluated. Finally, cytotoxicity was assessed using an extract test. The data were statistically analyzed using a one-way analysis of variance, followed by Tukey's multiple comparison test for post-hoc analysis.
RESULTS
The Sa value in the CENT group was lower than in the IPA, EtOH, TPM, and REF groups (p < 0.001). Moreover, the CENT group had lower Sq values than other groups (p < 0.001). The centrifugation method showed a higher FS value (80.92 ± 8.65 MPa) than the EtOH (61.71 ± 12.25 MPa, p < 0.001) and TPM (67.01 ± 9.751 MPa, p = 0.027), while affecting IPA (72.26 ± 8.80 MPa, p = 0.268) and REF (71.39 ± 10.44 MPa, p = 0.231). Also, the centrifugation method showed no evident cytotoxic effects.
CONCLUSIONS
The surfaces treated with a centrifugation method were relatively smooth. Simultaneously, the flexural strength of denture base polymers was enhanced through centrifugation. Finally, no evident cytotoxic effects could be observed from different post-processing procedures.
CLINICAL SIGNIFICANCE
The centrifugation method could optimize surface quality and flexural strength of DLP-printed denture base polymers without compromising cytocompatibility, offering an alternative to conventional rinsing post-processing.
目的
研究离心方法对增材制造义齿基托聚合物表面特性、弯曲性能和细胞毒性的影响。
方法
采用数字光处理(DLP)制备测试样本。使用离心方法(CENT)去除残留的未固化树脂。此外,分别使用不同的后冲洗溶液对样本进行后处理:异丙醇(IPA)、乙醇(EtOH)和三丙二醇单甲醚(TPM)。使用商业热聚合聚甲基丙烯酸甲酯作为对照(REF)。首先,测量表面形貌、算术平均高度(Sa)和均方根高度(Sq)值。然后,评估弯曲强度(FS)和模量。最后,采用浸提试验评估细胞毒性。使用单向方差分析对数据进行统计学分析,然后进行 Tukey 多重比较检验进行事后分析。
结果
CENT 组的 Sa 值低于 IPA、EtOH、TPM 和 REF 组(p < 0.001)。此外,CENT 组的 Sq 值低于其他组(p < 0.001)。离心法显示出更高的 FS 值(80.92 ± 8.65 MPa),高于 EtOH(61.71 ± 12.25 MPa,p < 0.001)和 TPM(67.01 ± 9.751 MPa,p = 0.027),但对 IPA(72.26 ± 8.80 MPa,p = 0.268)和 REF(71.39 ± 10.44 MPa,p = 0.231)无明显影响。此外,离心法未显示出明显的细胞毒性作用。
结论
经离心法处理的表面相对光滑。同时,通过离心法提高了义齿基托聚合物的弯曲强度。最后,不同后处理程序未观察到明显的细胞毒性作用。
临床意义
离心法可优化 DLP 打印义齿基托聚合物的表面质量和弯曲强度,同时不影响细胞相容性,为传统冲洗后处理提供了一种替代方法。