B Nirmala, Omar Balram J
Microbiology, All India Institute of Medical Sciences, Rishikesh, Rishikesh, IND.
Cureus. 2024 May 8;16(5):e59892. doi: 10.7759/cureus.59892. eCollection 2024 May.
Background Staphyloxanthin, a carotenoid pigment found in , serves not only to impart color but also functions as a crucial antioxidant contributing to virulence. Traditionally, milk agar has been employed to enhance staphyloxanthin production, however, no alternative media have been explored. Objectives This study aims to enhance staphyloxanthin production in using beetroot and carrot formulations. Methods To assess the efficacy of the media, we utilized filter paper, slide spot tests, and microscopic visualization as preliminary identification techniques. Ultraviolet-visible (UV-Vis) spectroscopy and paper chromatography were employed for characterization. Pigment quantification was conducted using microtiter plate assays, and genotypical detection was performed using Reverse Transcriptase-quantitative Polymerase Chain Reaction (RT-qPCR). Results Beetroot agar exhibited the highest pigment intensity, followed by beetroot with carrot agar, milk agar, carrot agar, and nutrient agar with the lowest intensity. These novel media formulations increased staphyloxanthin synthesis yield, resulting in spectrum shifts ranging from 450 nm (yellow) of milk agar to 470 nm (carrot agar) /480 nm (orange) of beetroot agar. Conclusion This study demonstrates that beetroot and carrot agar can effectively enhance staphyloxanthin production in . Furthermore, we propose the potential for large-scale cultivation of these pigments in future studies for various industrial applications, such as integration into paints, fabrics, and sunscreen lotions, due to their antioxidant properties.
背景 金黄色葡萄球菌黄素是一种存在于[具体对象未给出]中的类胡萝卜素色素，不仅用于赋予颜色，还作为一种关键的抗氧化剂，对毒力有贡献。传统上，乳琼脂一直被用于提高金黄色葡萄球菌黄素的产量，然而，尚未探索其他替代培养基。目的 本研究旨在利用甜菜根和胡萝卜配方提高[具体对象未给出]中金黄色葡萄球菌黄素的产量。方法 为了评估培养基的功效，我们使用滤纸、载玻片点滴试验和显微镜观察作为初步鉴定技术。采用紫外可见（UV-Vis）光谱和纸色谱进行表征。使用微量滴定板测定法进行色素定量，并使用逆转录定量聚合酶链反应（RT-qPCR）进行基因分型检测。结果 甜菜根琼脂显示出最高的色素强度，其次是甜菜根与胡萝卜琼脂、乳琼脂、胡萝卜琼脂，营养琼脂的色素强度最低。这些新型培养基配方提高了金黄色葡萄球菌黄素的合成产量，导致光谱从乳琼脂的450纳米（黄色）转移到甜菜根琼脂的470纳米（胡萝卜琼脂）/480纳米（橙色）。结论 本研究表明，甜菜根和胡萝卜琼脂可以有效提高[具体对象未给出]中金黄色葡萄球菌黄素的产量。此外，由于其抗氧化特性，我们提出在未来的研究中大规模培养这些色素用于各种工业应用的潜力，如融入涂料、织物和防晒乳液中。
