Bajpai Devika, Rajasekar Arvina
Periodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus. 2024 May 6;16(5):e59762. doi: 10.7759/cureus.59762. eCollection 2024 May.
Red clover, a perennial herbaceous plant, has been demonstrated to possess blood-purifying, expectorant, and calming properties. This research endeavors to create and evaluate the antimicrobial, antioxidant characteristics, and cytotoxic effects of the ethanolic extract derived from red clover.
A water-based solution of red clover was formulated and subjected to centrifugation. Various concentrations of the extract were applied to the wells of agar plates inoculated with and and then left to incubate. The inhibition zones for each concentration were subsequently measured. The antioxidant properties were evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, while the cytotoxicity of the extract was assessed through the brine shrimp lethality assay.
Initially, the extract was tested with a volume of 10 μL, which was subsequently incremented to 20 μL, 30 μL, 40 μL, and 50 μL. According to the DPPH assay, as the concentration of the extract solution increased incrementally by 10 μL, its antioxidant activity also exhibited a corresponding rise. The cytotoxicity assay indicated that the mouthwash formulated with red clover had minimal cytotoxic effects within the range of 5-20 µL. Antibacterial analysis revealed a similar zone of inhibition between the test and control groups.
The ethanolic extract obtained from red clover was identified as a powerful antioxidant, antibacterial, and biocompatible substance. Hence, it can be a potential candidate for application as a mouthwash.
红三叶草是一种多年生草本植物，已被证明具有净化血液、祛痰和平静的特性。本研究旨在制备并评估红三叶草乙醇提取物的抗菌、抗氧化特性及细胞毒性作用。
配制红三叶草的水溶液并进行离心。将不同浓度的提取物应用于接种了[具体菌种1]和[具体菌种2]的琼脂平板孔中，然后进行培养。随后测量每种浓度的抑菌圈。使用2,2-二苯基-1-苦基肼基（DPPH）法评估抗氧化性能，通过卤虫致死试验评估提取物的细胞毒性。
最初，提取物以10μL的体积进行测试，随后依次增加到20μL、30μL、40μL和50μL。根据DPPH法，随着提取物溶液浓度以10μL的增量递增，其抗氧化活性也相应提高。细胞毒性试验表明，用红三叶草配制的漱口水在5 - 20μL范围内细胞毒性极小。抗菌分析显示试验组和对照组之间的抑菌圈相似。
从红三叶草中获得的乙醇提取物被鉴定为一种强大的抗氧化、抗菌且生物相容性良好的物质。因此，它有可能成为漱口水应用的潜在候选物。
