Gattone V H, Filo R S, Evan A P, Leapman S B, Smith E J, Luft F C
Transplantation. 1985 Apr;39(4):396-9. doi: 10.1097/00007890-198504000-00012.
To elucidate the time course of glomerular and arterial endothelial injury resulting from pulsatile perfusion preservation of human kidneys, we examined two kidneys, one at 16 and the other at 42 hr, for which no suitable recipient could be found. The scanning electron microscope revealed subtle changes at 16 hr in the filtration barrier. These included mild endothelial swelling with an increase in the appearance of bulbous processes, and elongated fenestrae. The visceral epithelial surface was normal as was the arterial endothelial surface. By 42 hr the glomerular endothelial surface displayed very prominent cytoplasmic ridges and clearly distorted fenestrae. The arterial endothelium exhibited a tendency to separate from the vessel wall. The proximal tubular epithelium revealed scattered loss of microvilli. These changes are similar in kind to, albeit less severe than, those described after 60 hr of perfusion. They may represent cell swelling following ischemia, or be the result of altered cell permeability engendered by low temperature. The possibility remains that such changes could be minimized by modifying the perfusate. Scanning electron microscopy provides a versatile tool in the study of vascular and other surfaces of tissues stored with perfusion preservation.
为了阐明人肾搏动灌注保存所导致的肾小球和动脉内皮损伤的时间进程，我们检查了两个肾脏，一个在16小时时检查，另一个在42小时时检查，这两个肾脏均未找到合适的受者。扫描电子显微镜显示，在16小时时滤过屏障出现细微变化。这些变化包括轻度内皮肿胀，球茎状突起外观增加，以及窗孔拉长。脏层上皮表面正常，动脉内皮表面也正常。到42小时时，肾小球内皮表面出现非常突出的细胞质嵴，窗孔明显扭曲。动脉内皮显示出与血管壁分离的趋势。近端肾小管上皮显示微绒毛散在丢失。这些变化在类型上与灌注60小时后描述的变化相似，尽管程度较轻。它们可能代表缺血后的细胞肿胀，或者是低温导致的细胞通透性改变的结果。通过改变灌注液，这种变化有可能被最小化。扫描电子显微镜为研究灌注保存的组织的血管和其他表面提供了一种通用工具。
