Chamsai Ekkapat, Charoenkul Kamonpan, Udom Kitikhun, Jairak Waleemas, Chaiyawong Supassama, Amonsin Alongkorn
Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals, and One Health Research Cluster, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Front Vet Sci. 2024 May 24;11:1415771. doi: 10.3389/fvets.2024.1415771. eCollection 2024.
Rotavirus A (RVA) causes gastroenteritis in humans and animals. The zoonotic potential of RVA has been reported and raises major concerns, especially in animal-human interface settings. The study aimed to characterize and investigate the genetic diversity among RVAs in dogs and cats in Thailand. We collected 572 rectal swab samples from dogs and cats in Bangkok animal hospitals from January 2020 to June 2021. The one-step RT-PCR assay detected RVAs in 1.92% (11/572) of the samples, with 2.75% (8/290) in dogs and 1.06% (3/282) in cats. Two canine RVA and one feline RVA were subjected to whole genome sequencing. Our results showed that all three viruses were identified as RVA genotype G3P[3]. The genetic constellation of RVAs is unique for different species. For canine RVAs is G3-P [3]-I3-R3-C3-M3-A9-N2-T3-E3-H6, while Feline RVA is G3-P [3]-I8-R3-C3-M3-A9-N3-T3-E3-H6. Notably, both canine and feline RVAs contained the AU-1 genetic constellation with multiple reassortments. The results of phylogenetic, genetic, and bootscan analyses showed that canine RVAs may have reassorted from dog, human, and cat RVAs. While feline RVA was closely related to RVAs in humans, bats, and simians. This study provided genetic characteristics and diversity of RVAs in dogs and cats and suggested possible multiple reassortments, suggesting the zoonotic potential of the viruses. Thus, public health awareness should be raised regarding the zoonotic potential of RVAs in dogs and cats. Further studies on RVAs on a larger scale in dogs and cats in Thailand are needed.
A组轮状病毒（RVA）可引起人和动物的肠胃炎。RVA的人畜共患病潜力已被报道，这引发了人们的重大担忧，尤其是在动物与人类接触的环境中。该研究旨在对泰国犬猫RVA的基因多样性进行表征和调查。我们于2020年1月至2021年6月从曼谷动物医院的犬猫身上收集了572份直肠拭子样本。一步法RT-PCR检测在1.92%（11/572）的样本中检测到RVA，其中犬样本中为2.75%（8/290），猫样本中为1.06%（3/282）。对两株犬RVA和一株猫RVA进行了全基因组测序。我们的结果表明，这三种病毒均被鉴定为RVA G3P[3]基因型。RVA的基因组合对于不同物种而言是独特的。犬RVA的基因组合为G3-P [3]-I3-R3-C3-M3-A9-N2-T3-E3-H6，而猫RVA的基因组合为G3-P [3]-I8-R3-C3-M3-A9-N3-T3-E3-H6。值得注意的是，犬和猫的RVA均包含具有多个重配的AU-1基因组合。系统发育、遗传和Bootscan分析结果表明，犬RVA可能是由犬、人和猫的RVA重配而来。而猫RVA与人类、蝙蝠和猿猴的RVA密切相关。本研究提供了犬猫RVA的遗传特征和多样性，并提示可能存在多次重配，这表明这些病毒具有人畜共患病潜力。因此，应提高公众对犬猫RVA人畜共患病潜力的认识。泰国需要对犬猫RVA进行更大规模的进一步研究。
