Dong Huijin, Qian Yuan, Nong Yi, Zhang You, Mo Zhaojun, Li Rongcheng
Bing Du Xue Bao. 2016 Mar;32(2):129-40.
One unusual human G3P[3] group A rotavirus (RVA) strain M2-102 was identified in stool sample collected from a child with diarrhea in Guangxi Province, China in 2014. It is well known that G3P[3] is a genotype commonly identified in feline and canine RVAs. However, the preliminary phylogenetic analyses of the VP7 and VP4 genes of strain M2-102 indicated that these two genes were closely related to bat RVA strain MYAS33 and simian strain RRV, respectively, whereas both clustered distantly to feline/canine-like RVA strains. In this study, full genome sequencing and molecular analyses were conducted to obtain the true origin of strain M2-102. It was revealed that strain RVA/Human-wt/CHN/M2-102/2014/G3P[3] exhibited a G3-P[3]-I3-R3-C3-M3-A9-N3-T3-E3-H6 genotype constellation for VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes. Phylogenetic analyses revealed that 5 genes (VP7, VP1, VP2, NSP2 and NSP3) from strain M2-102 were closely related to those of bat strain MYAS33 from Yunnan Province which was thought a true bat RVA strain rather than a virus transmitted between species, while another 5 genes (VP4, VP3, NSP1, NSP4 and NSP5) clustered closely with those of simian strain RRV, yet the VP6 gene was closely related to that of human G3P[9] strain AU-1 and AU-1-like RVAs. The epidemiological data indicated that the child infected with M2-102 came from a countryside village, located in Dong Autonomous County of Sanjiang (subtropical hilly wooded area), Liuzhou city in Guangxi Province which might provide natural environment for reassortment events occurring among animal and human RVAs. Therefore, the data suggest that human strain M2-102 might originate from multiple reassortment events among bat, simian and human AU-1-like RVAs, yet it is not clear whether the genomic backbone based on bat MYAS33 (5 genes) and simian RRV (5 genes) like rotaviruses had been obtained through reassortment before being transmitted to the human. This is the first report on whole genome analysis of human G3P[3] RVA from China.
2014年，在中国广西省一名腹泻儿童的粪便样本中，鉴定出一株不寻常的人G3P[3] A组轮状病毒（RVA）毒株M2-102。众所周知，G3P[3]是在猫和犬RVA中常见的一种基因型。然而，对毒株M2-102的VP7和VP4基因进行的初步系统发育分析表明，这两个基因分别与蝙蝠RVA毒株MYAS33和猿猴毒株RRV密切相关，而它们与猫/犬样RVA毒株的聚类关系较远。在本研究中，进行了全基因组测序和分子分析以确定毒株M2-102的真正来源。结果显示，毒株RVA/Human-wt/CHN/M2-102/2014/G3P[3]在VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5基因上呈现出G3-P[3]-I3-R3-C3-M3-A9-N3-T3-E3-H6基因型组合。系统发育分析表明，毒株M2-102的5个基因（VP7、VP1、VP2、NSP2和NSP3）与来自云南省的蝙蝠毒株MYAS33的基因密切相关，MYAS33被认为是一株真正的蝙蝠RVA毒株，而非种间传播的病毒，而另外5个基因（VP4、VP3、NSP1、NSP4和NSP5）与猿猴毒株RRV的基因聚类关系密切，不过VP6基因与人类G3P[9]毒株AU-1和AU-1样RVA的VP6基因密切相关。流行病学数据表明，感染M2-102的儿童来自广西柳州市三江侗族自治县（亚热带丘陵林区）的一个乡村，这可能为动物和人类RVA之间发生的重配事件提供了自然环境。因此，数据表明人毒株M2-102可能起源于蝙蝠、猿猴和人类AU-1样RVA之间的多次重配事件，但尚不清楚基于蝙蝠MYAS33（5个基因）和猿猴RRV（5个基因）的轮状病毒基因组主干在传播给人类之前是否通过重配获得。这是中国关于人G3P[3] RVA全基因组分析的首次报道。
