用于多光子显微镜中烟酰胺腺嘌呤二核苷酸（NADH）荧光检测的高选择性光学滤波器。
Highly-selective optical filter for NADH fluorescence detection in multiphoton microscopy.
作者信息
Freitas R B, Rodrigues M J L F, Pimenta S, Belsley M, Correia J H, Maciel M J
机构信息
CMEMS-UMinho, University of Minho, 4800-058, Guimarães, Portugal.
Centre of Physics of Minho and Porto Universities (CF-UM-UP), Laboratory for Materials and Emergent Technologies (LAPMET), University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
出版信息
Biomed Opt Express. 2024 Apr 24;15(5):3317-3328. doi: 10.1364/BOE.506777. eCollection 2024 May 1.
Colorectal cancer (CRC) is a pressing global health concern, emphasizing the need for early detection tools. In this study an optical filter for precise detection of nicotinamide adenine dinucleotide (NADH) fluorescence via two-photon excitation fluorescence (TPEF) was developed. Fabricated with silicon dioxide and titanium dioxide thin films in a Fabry-Perot structure, the filter achieved a peak transmittance of about 95% at 483 nm, with a 12 nm full-width at half maximum. TPEF measurements using a tailored setup and NADH liquid phantoms underscored the filter's significance in selectively capturing NADH fluorescence while mitigating interference from other fluorophores. This work marks a substantial stride towards integrating multiphoton microscopy into conventional colonoscopy, enabling non-invasive, objective optical biopsy for colorectal tissue analysis. Further refinements of the experimental setup are imperative to advance tissue differentiation and enhance CRC diagnosis.
结直肠癌（CRC）是一个紧迫的全球健康问题，这凸显了对早期检测工具的需求。在本研究中，开发了一种用于通过双光子激发荧光（TPEF）精确检测烟酰胺腺嘌呤二核苷酸（NADH）荧光的光学滤波器。该滤波器采用二氧化硅和二氧化钛薄膜制成法布里-珀罗结构，在483 nm处实现了约95%的峰值透过率，半高宽为12 nm。使用定制装置和NADH液体模型进行的TPEF测量强调了该滤波器在选择性捕获NADH荧光同时减轻其他荧光团干扰方面的重要性。这项工作标志着在将多光子显微镜集成到传统结肠镜检查方面迈出了重要一步，能够对结直肠组织进行非侵入性、客观的光学活检分析。进一步完善实验装置对于推进组织分化和提高CRC诊断至关重要。
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