利用多光子荧光显微镜进行深层组织成像。

Miller David R, Jarrett Jeremy W, Hassan Ahmed M, Dunn Andrew K

Department of Biomedical Engineering, The University of Texas at Austin, 107 W. Dean Keeton C0800, Austin, TX 78712, USA.

Curr Opin Biomed Eng. 2017 Dec;4:32-39. doi: 10.1016/j.cobme.2017.09.004. Epub 2017 Sep 27.

We present a review of imaging deep-tissue structures with multiphoton microscopy. We examine the effects of light scattering and absorption due to the optical properties of biological sample and identify 1,300 nm and 1,700 nm as ideal excitation wavelengths. We summarize the availability of fluorophores for multiphoton microscopy as well as ultrafast laser sources to excite available fluorophores. Lastly, we discuss the applications of multiphoton microscopy for neuroscience.

我们对利用多光子显微镜成像深层组织结构进行了综述。我们研究了由于生物样品的光学特性导致的光散射和吸收效应，并确定1300纳米和1700纳米为理想的激发波长。我们总结了用于多光子显微镜的荧光团以及用于激发可用荧光团的超快激光源的可用性。最后，我们讨论了多光子显微镜在神经科学中的应用。