Wang Dan, Lin Zhiqiang, Su Meixia, Zhou Yiqing, Ma Mengjie, Li Minghui
Shaoxing People's Hospital, Department of Infectious Diseases, Shaoxing, Zhejiang, People's Republic of China.
Shaoxing People's Hospital, Department of general surgery, Shaoxing, Zhejiang, People's Republic of China.
Genet Mol Biol. 2024 May 31;47(2):e20230205. doi: 10.1590/1678-4685-GMB-2023-0205. eCollection 2024.
To investigate the role of Peg13 in modulating the inflammatory response in sepsis, we established Lipopolysaccharide (LPS)-induced 293T cells and mouse models. Peg13 expression was assessed at various time points after infection using RT-qPCR. The levels of high mobility group box 1 (HMGB1) and interleukin-6 (IL-6) were quantified through ELISA. A total of 44 septic patients and 36 healthy participants were recruited to measure Peg13 and HMGB1 levels in the blood. Peg13 demonstrated significant down-regulation in the supernatant of LPS-induced 293T cells and in the blood of LPS-induced mice. Moreover, the levels of proinflammatory cytokines HMGB1 and IL-6 were elevated in both the supernatant of LPS-induced cell models and blood specimens from LPS-induced murine models, and this elevation could be notably reduced by Peg13 suppression. In a clinical context, Peg13 and HMGB1 levels were higher in septic patients compared to healthy subjects. Peg13 exhibited a negative correlation with HMGB1, C-reactive protein (CRP), and erythrocyte sedimentation rate (ESR) among septic patients. Peg13 mitigates the inflammatory response by reducing the release of proinflammatory cytokines HMGB1 and IL-6 in sepsis, presenting a potential therapeutic target for alleviating inflammation in sepsis treatment.
为了研究Peg13在调节脓毒症炎症反应中的作用，我们建立了脂多糖（LPS）诱导的293T细胞模型和小鼠模型。使用RT-qPCR在感染后的不同时间点评估Peg13的表达。通过ELISA定量检测高迁移率族蛋白B1（HMGB1）和白细胞介素-6（IL-6）的水平。共招募了44例脓毒症患者和36名健康参与者来检测血液中Peg13和HMGB1的水平。Peg13在LPS诱导的293T细胞上清液和LPS诱导的小鼠血液中均表现出显著下调。此外，LPS诱导的细胞模型上清液和LPS诱导的小鼠模型血液标本中促炎细胞因子HMGB1和IL-6的水平均升高，而Peg13抑制可显著降低这种升高。在临床环境中，脓毒症患者的Peg13和HMGB1水平高于健康受试者。脓毒症患者中，Peg13与HMGB1、C反应蛋白（CRP）和红细胞沉降率（ESR）呈负相关。Peg13通过减少脓毒症中促炎细胞因子HMGB1和IL-6的释放来减轻炎症反应，为脓毒症治疗中的炎症缓解提供了一个潜在的治疗靶点。
