Bone formation enhanced by induction. Bone growth in titanium implants in rats.

Bone-inducing materials have been investigated for the purpose of augmenting bone formation in implants made of porous fiber titanium. The bone-inducing materials used were: (1) Bone from the iliac crest of inbred rats (isografts), (2) Antigen-extracted, autolyzed, demineralized bone from outbred rats (AAA bone a.m. Urist), and (3) AAA bone combined with bone marrow from inbred rats. Tubes of fiber titanium were packed with bone-inducing materials and implanted in the back musculature of inbred rats. Bone formation was assessed by labelling with 3H-proline (collagen synthesis) and 47Ca (mineral deposit) and by content of calcium of the harvested implants. Isografts and AAA bone with marrow yielded a substantial amount of new bone. Without the marrow, AAA bone yielded very small amounts of new bone.