Department of Plant and Environmental Science, Clemson University, 105 Collings Street, Clemson, SC 29634, U.S.A.
Hubei Key Lab of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Phytopathology. 2024 Sep;114(9):2064-2070. doi: 10.1094/PHYTO-03-24-0090-R. Epub 2024 Aug 20.
Mixtures of fungicides with different modes of action are commonly used as disease and resistance management tools, but little is known of mixtures of natural and synthetic products. In this study, mixtures of metabolites from the rhizobacterium strain ASF009 formulated as Howler EVO with below-label rates (50 µg/ml) of conventional sterol demethylation inhibitor (DMI) fungicides were investigated for control of anthracnose of cherry () caused by . Howler mixed with metconazole or propiconazole synergistically reduced disease severity through lesion growth. Real-time PCR showed that difenoconazole, flutriafol, metconazole, and propiconazole induced the expression of DMI target genes and in . The addition of Howler completely suppressed the DMI fungicide-induced expression of both genes. We hypothesize that the downregulation of DMI fungicide-induced expression of the DMI target genes may, at least in part, explain the synergism observed in detached fruit assays.
将根际细菌菌株 ASF009 的代谢产物制成的制剂 Howler EVO 与常规甾醇脱甲基抑制剂 (DMI) 类杀菌剂以低于标签浓度（50μg/ml）混合，用于防治由 引起的樱桃炭疽病（）。Howler 与咪鲜胺或丙环唑混合使用通过抑制病斑生长表现出增效作用，降低病害严重度。实时 PCR 显示，氟环唑、咯菌腈、咪鲜胺和丙环唑诱导 DMI 类杀菌剂靶标基因 和 在 中的表达。Howler 的添加完全抑制了 DMI 类杀菌剂诱导的这两个基因的表达。我们假设 DMI 类杀菌剂靶标基因表达的下调可能至少部分解释了在离体果实测定中观察到的增效作用。
