Karami Pari, Gholamin Danial, Fathi Farzaneh, Afsar Taha, Johari-Ahar Mohammad
Biosensor Sciences and Technologies Research Center, Ardabil University of Medical Sciences, Ardabil, Iran.
Biosensor Sciences and Technologies Research Center, Ardabil University of Medical Sciences, Ardabil, Iran; Department of Medicinal Chemistry and Pharmacognosy, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
Clin Chim Acta. 2024 Jul 15;561:119767. doi: 10.1016/j.cca.2024.119767. Epub 2024 Jun 8.
Biosensors are unique analytical tools for the detection of biomarkers. Of these, autoantibodies against citrullinated proteins (ACPA) are useful for the differential diagnosis of rheumatoid arthritis (RA). The autoantibodies may be detected by immunoassay technology using synthetic cyclic citrullinated peptides (CCP), ie, anti-CCP. Recently, several biosensors have been developed for anti-CCP using CCP and mutated citrullinated vimentin (MCV) as recognition elements. In this review we highlight all currently available ACPA biosensor technology including those based on fluorescence, chemiluminescence, electrochemiluminescence (ECL), surface-enhanced Raman scattering (SERS)-based, surface plasmon resonance (SPR), lateral flow immunoassays (LFIA), and electrochemical. We explore various peptides as recognition elements, electrode modifiers and signal amplification systems thus providing new opportunities for next-generation biosensor design in RA.
生物传感器是用于检测生物标志物的独特分析工具。其中，抗瓜氨酸化蛋白自身抗体（ACPA）对类风湿性关节炎（RA）的鉴别诊断很有用。这些自身抗体可以通过使用合成环瓜氨酸化肽（CCP）的免疫测定技术来检测，即抗CCP。最近，已经开发了几种以CCP和突变的瓜氨酸化波形蛋白（MCV）作为识别元件的用于检测抗CCP的生物传感器。在这篇综述中，我们重点介绍了所有当前可用的ACPA生物传感器技术，包括基于荧光、化学发光、电化学发光（ECL）、表面增强拉曼散射（SERS）、表面等离子体共振（SPR）、侧向流动免疫分析（LFIA）和电化学的技术。我们探索了各种肽作为识别元件、电极修饰剂和信号放大系统，从而为RA的下一代生物传感器设计提供了新的机遇。
