Dong Feng, Wang Limin
Department of Laboratory, Jinhua Maternal and Child Health Hospital, Jinhua, 321000, China.
Department of Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, No. 365, Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang Province, China.
Clin Exp Med. 2025 Aug 24;25(1):302. doi: 10.1007/s10238-025-01850-5.
Rheumatoid arthritis (RA) is a persistent autoimmune disorder where serological biomarkers play a crucial role in diagnosis and monitoring disease activity. Antibodies targeting cyclic citrullinated peptides (anti-CCP), mutated citrullinated vimentin (anti-MCV), and rheumatoid factor are commonly used serological markers for RA. However, their respective diagnostic efficacies and potential for mutual complementation remain incompletely understood. This study investigates the diagnostic performance of these three antibodies and their association with disease progression in RA. A total of 257 RA patients who visited Jinhua Hospital Affiliated with Zhejiang University between March and December 2019 were enrolled. Serum specimens were analyzed for anti-CCP, anti-MCV antibodies, and RF levels using chemiluminescence immunoassay (CLIA) and rate nephelometry. The results indicated that the specificity of anti-CCP (94.2%) was higher than that of anti-MCV (84.4%) and RF (84.8%). Furthermore, anti-MCV antibody levels were significantly link to disease duration and morning stiffness. Additionally, anti-MCV and anti-CCP demonstrated differing associations with extra-articular manifestations of RA. The study suggests that anti-MCV antibodies hold significant potential as adjunctive biomarkers in RA, complementing anti-CCP antibodies to improve diagnostic accuracy and provide new insights for early diagnosis and disease monitoring in RA.
类风湿关节炎（RA）是一种持续性自身免疫性疾病，血清生物标志物在其诊断和疾病活动监测中起着关键作用。靶向环瓜氨酸肽的抗体（抗CCP）、突变瓜氨酸波形蛋白抗体（抗MCV）和类风湿因子是常用于RA的血清学标志物。然而，它们各自的诊断效能以及相互补充的潜力仍未完全明确。本研究调查了这三种抗体的诊断性能及其与RA疾病进展的关联。共纳入了2019年3月至12月期间就诊于浙江大学附属金华医院的257例RA患者。采用化学发光免疫分析法（CLIA）和速率散射比浊法分析血清标本中的抗CCP、抗MCV抗体及RF水平。结果表明，抗CCP的特异性（94.2%）高于抗MCV（84.4%）和RF（84.8%）。此外，抗MCV抗体水平与疾病持续时间和晨僵显著相关。另外，抗MCV和抗CCP与RA的关节外表现存在不同的关联。该研究表明，抗MCV抗体作为RA的辅助生物标志物具有巨大潜力，可与抗CCP抗体互补以提高诊断准确性，并为RA的早期诊断和疾病监测提供新的见解。
