Department of Biomedicine, Atta-ur-Rehman School of Applied Biosciences, National University of Sciences & Technology, Sector H-12, 44000 Islamabad, Pakistan; Department of Biological Sciences, National University of Medical Sciences, Abid Majeed Road, 46000 Rawalpindi, Pakistan.
Department of Biological Sciences, National University of Medical Sciences, Abid Majeed Road, 46000 Rawalpindi, Pakistan.
Int J Biol Macromol. 2024 Jul;273(Pt 1):133030. doi: 10.1016/j.ijbiomac.2024.133030. Epub 2024 Jun 8.
Skin wound healing and regeneration is very challenging across the world as simple or acute wounds can be transformed into chronic wounds or ulcers due to foreign body invasion, or diseases like diabetes or cancer. The study was designed to develop a novel bioactive scaffold, by loading aloesin to chitosan-coated cellulose scaffold, to cure full-thickness skin wounds. The physiochemical characterization of the scaffold was carried out using scanning electron microscopy (SEM) facilitated by energy-dispersive spectrophotometer (EDS), atomic force microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR). The results indicated the successful coating of chitosan and aloesin on cellulose without any physical damage. The drug release kinetics confirmed the sustained release of aloesin by showing a cumulative release of up to 88 % over 24 h. The biocompatibility of the aloesin-loaded chitosan/cellulose (AlCsC) scaffold was evaluated by the WST-8 assay that confirmed the significantly increased adherence and proliferation of fibroblasts on the AlCsC scaffold. The in vivo wound healing study showed that both 0.05 % and 0.025 % AlCsC scaffolds have significantly higher wound closure rates (i.e. 88.2 % and 95.6 % approximately) as compared to other groups. This showed that novel composite scaffold has a wound healing ability. Furthermore, histological and gene expression analysis demonstrated that the scaffold also induced cell migration, angiogenesis, re-epithelialization, collagen deposition, and tissue granulation formation. Thus, it is concluded that the aloesin-loaded chitosan/cellulose-based scaffold has great therapeutic potential for being used in wound healing applications in the clinical setting in the future.
皮肤创伤愈合和再生在全球范围内极具挑战性，因为简单或急性创伤会由于异物入侵或糖尿病、癌症等疾病而转变为慢性创伤或溃疡。本研究旨在开发一种新型生物活性支架，通过将艾酸负载到壳聚糖涂覆的纤维素支架上，以治愈全层皮肤创伤。通过扫描电子显微镜（SEM）结合能谱仪（EDS）、原子力显微镜（AFM）和傅里叶变换红外光谱（FTIR）对支架的物理化学特性进行了表征。结果表明，壳聚糖和艾酸成功地涂覆在纤维素上，没有任何物理损伤。药物释放动力学证实了艾酸的持续释放，在 24 小时内累积释放高达 88%。通过 WST-8 测定评估了载艾酸壳聚糖/纤维素（AlCsC）支架的生物相容性，证实了成纤维细胞在 AlCsC 支架上的粘附和增殖显著增加。体内创伤愈合研究表明，0.05%和 0.025%的 AlCsC 支架的创面闭合率明显更高（分别约为 88.2%和 95.6%），与其他组相比。这表明新型复合支架具有创伤愈合能力。此外，组织学和基因表达分析表明，支架还诱导了细胞迁移、血管生成、再上皮化、胶原沉积和组织肉芽形成。因此，可以得出结论，载艾酸壳聚糖/纤维素基支架具有很大的治疗潜力，可用于未来临床环境中的创伤愈合应用。
