QOPNA Research Unit, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Int J Biol Macromol. 2012 Nov;51(4):343-50. doi: 10.1016/j.ijbiomac.2012.05.023. Epub 2012 May 28.
The purpose of this study was to evaluate hybrid poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/chitosan nanofibrous mats as scaffolds for skin engineering. In vitro studies were carried out to test the potential of the scaffolds for fibroblasts adhesion, viability, and proliferation (L929 cell line). The in vivo performance was also studied in a full-thickness wound healing model. PHBV/chitosan 4:1 (w/w) exhibited a higher in vitro biocompatibility and a better ability for cell adhesion and growth, compared to PHBV/chitosan 2:3 (w/w). The in vivo assay also revealed the better performance of this scaffold, improving the wound healing process in rats.
本研究旨在评估混合聚(3-羟基丁酸酯-共-3-羟基戊酸酯) (PHBV)/壳聚糖纳米纤维垫作为皮肤工程支架。进行了体外研究以测试支架对成纤维细胞黏附、活力和增殖（L929 细胞系）的潜力。还在全层创面愈合模型中研究了体内性能。与 PHBV/壳聚糖 2:3（w/w）相比，PHBV/壳聚糖 4:1（w/w）表现出更高的体外生物相容性和更好的细胞黏附和生长能力。体内试验还揭示了该支架的更好性能，改善了大鼠的伤口愈合过程。
