Department of Zoology, Faculty of Science, Al-Azhar University (Assiut branch), Assiut 71524, Egypt; Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Skip Bertman Drive, Baton Rouge, LA 70803, USA.
Center for Environmental and Human Toxicology, Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32611, USA.
Environ Toxicol Pharmacol. 2024 Aug;109:104481. doi: 10.1016/j.etap.2024.104481. Epub 2024 Jun 8.
Pyrogallol, a botanical hydrolysable tannin, has diverse medical and industrial applications. Its impact on aquatic ecosystems and fish health has been previously studied, revealing histopathological, immunological, biochemical, and haematological alterations in African catfish (Clarias gariepinus). In this study, the neurotoxic potential of pyrogallol was assessed through a 15-day exposure of catfish to concentrations of 1, 5, or 10 mg/L. Enzyme activities such as acetylcholinesterase (AchE), monoamine oxidase (MAO), aldehyde oxidase (AO), and nitric oxide (NO) were measured in serum and brain, along with histopathological examinations in the brain and heart. Pyrogallol exposure led to decreased AchE activity in the brain and serum, increased serum MAO activity, elevated AO in both brain and serum, and suppressed NO levels. Morphological abnormalities and dose-dependent pathological alterations were observed in the brain and heart, including neuropile deformities, shrunken Purkinje cells, cardiomyocyte degeneration, and increased collagen fibers. This suggests that pyrogallol induces adverse effects in fish.
焦性没食子酸是一种植物性可水解单宁，具有多种医学和工业应用。以前已经研究过它对水生生态系统和鱼类健康的影响，结果表明非洲鲶鱼（Clarias gariepinus）的组织病理学、免疫学、生物化学和血液学发生了变化。在这项研究中，通过将鲶鱼暴露于 1、5 或 10mg/L 的浓度下 15 天，评估了焦性没食子酸的神经毒性潜力。在血清和脑中测量了酶活性，如乙酰胆碱酯酶（AchE）、单胺氧化酶（MAO）、醛氧化酶（AO）和一氧化氮（NO），并对脑和心脏进行了组织病理学检查。焦性没食子酸暴露导致脑和血清中的 AchE 活性降低，血清中 MAO 活性增加，脑和血清中的 AO 升高，NO 水平降低。在大脑和心脏中观察到形态异常和剂量依赖性的病理改变，包括神经毡变形、浦肯野细胞缩小、心肌细胞变性和胶原纤维增加。这表明焦性没食子酸对鱼类有不良影响。
