Lutz L H, Yayanos A A
Anal Biochem. 1985 Jan;144(1):1-5. doi: 10.1016/0003-2697(85)90075-2.
A spectrofluorometric technique for bacterial DNA base composition has been developed. This fast and simple technique requires two fluorescent dyes and a few inexpensive reagents. The data from this assay indicate that the guanine-cytosine content obtained was within acceptable statistical limits in comparison to commonly cited literature values. The spectrofluorometric technique is reliable and reproducible.
一种用于细菌DNA碱基组成的荧光分光光度技术已被开发出来。这种快速且简单的技术需要两种荧光染料和一些便宜的试剂。该检测方法得到的数据表明,与常见文献值相比,所获得的鸟嘌呤-胞嘧啶含量在可接受的统计范围内。这种荧光分光光度技术可靠且可重复。