Spectropolarimetric determination of the guanine-cytosine content of DNA.
作者信息
Maiti M, Nandi R
机构信息
Indian Institute of Chemical Biology, Calcutta.
出版信息
Anal Biochem. 1987 Jul;164(1):68-71. doi: 10.1016/0003-2697(87)90368-x.
PMID:3674372
Abstract
A circular dichroism technique for the determination of DNA base composition in terms of the guanine-cytosine content has been developed. A linear relationship between the ratio of ellipticities at 260 and 280 nm (theta 260/theta 280) and base composition of DNA is obtained in citrate-phosphate buffer, pH 3.0 at 20 degrees C. The DNA base composition obtained with this method is not only highly reproducible but is also the fastest method reported.
摘要
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