New England Biolabs Incorporated, Ipswich, Massachusetts 01938, USA.
SLC Management, Wellesley Hills, Massachusetts 02481, USA.
Genome Res. 2024 Jul 23;34(6):904-913. doi: 10.1101/gr.278294.123.
Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome.
多组学需要协同记录独立的信息，理想情况下是来自单个实验。在这项研究中，我们介绍了 RIMS-seq2，这是一种高通量技术，可以同时对基因组进行测序，并覆盖甲基化信息，而只需要对高通量 DNA 测序的实验方案进行微小修改，包括一个受控脱氨步骤。重要的是，5-甲基胞嘧啶的脱氨率可以忽略不计，因此不会干扰标准的 DNA 测序和数据处理。因此，与标准 DNA-seq 相比，来自全基因组或靶向基因组测序的 RIMS-seq2 文库具有相同的种系变异调用准确性和敏感性。此外，区域甲基化水平提供了人类甲基组的精确图谱。
