Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA), Institute of Biomedical Sciences, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain.
Institute for Animal Science and Technology, Universitat Politècnica de València, Valencia, Spain.
Commun Biol. 2024 Jun 10;7(1):712. doi: 10.1038/s42003-024-06322-2.
With the main aim of identifying biomarkers that contribute to defining the concept of ideal protein in growing rabbits under the most diverse conditions possible this work describes two different experiments. Experiment 1: 24 growing rabbits are included at 56 days of age. The rabbits are fed ad libitum one of the two experimental diets only differing in lysine levels. Experiment 2: 53 growing rabbits are included at 46 days of age, under a fasting and eating one of the five experimental diets, with identical chemical composition except for the three typically limiting amino acids (being fed commercial diets ad libitum in both experiments). Blood samples are taken for targeted and untargeted metabolomics analysis. Here we show that the metabolic phenotype undergoes alterations when animals experience a rapid dietary shift in the amino acid levels. While some of the differential metabolites can be attributed directly to changes in specific amino acids, creatinine, urea, hydroxypropionic acid and hydroxyoctadecadienoic acid are suggested as a biomarker of amino acid imbalances in growing rabbits' diets, since its changes are not attributable to a single amino acid. The fluctuations in their levels suggest intricate amino acid interactions. Consequently, we propose these metabolites as promising biomarkers for further research into the concept of the ideal protein using rabbit as a model.
本研究的主要目的是鉴定有助于定义在尽可能多样化的条件下生长兔的理想蛋白概念的生物标志物。为此进行了两项不同的实验。实验 1：在 56 日龄时纳入 24 只生长兔，自由采食两种实验日粮中的一种，两种日粮仅在赖氨酸水平上存在差异。实验 2：在 46 日龄时纳入 53 只生长兔，禁食并摄入五种实验日粮中的一种，除三种典型的限制氨基酸外，五种日粮的化学组成完全相同（两种实验中均自由采食商业日粮）。采集血液样本进行靶向和非靶向代谢组学分析。结果表明，当动物经历氨基酸水平的快速膳食变化时，代谢表型会发生改变。虽然一些差异代谢物可以直接归因于特定氨基酸的变化，但肌酸酐、尿素、羟基丙酸和羟基十八碳二烯酸被认为是生长兔日粮中氨基酸失衡的生物标志物，因为其变化不能归因于单一氨基酸。它们水平的波动表明存在复杂的氨基酸相互作用。因此，我们提出这些代谢物作为进一步研究兔模型理想蛋白概念的有前途的生物标志物。
