Marín-García Pablo Jesús, García-Párraga Daniel, Crespo-Picazo Jose Luis, Llobat Lola, Cambra-López María, Bordignon Francesco, Pascual Juan José, Larsen Torben, Hedemann Mette Skou
Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA), Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, Spain.
Fundación Oceanogràfic de la Comunitat Valenciana, Ciudad de las Artes y las Ciencias, 46013 Valencia, Spain.
Int J Mol Sci. 2025 Jan 10;26(2):545. doi: 10.3390/ijms26020545.
To investigate the impact of age on the metabolomic profile of loggerhead sea turtles (), this study analyzed 100 plasma samples of individuals across two age groups-50 post-hatchlings and 50 juveniles-from various locations along the Mediterranean coastline. Both targeted and untargeted metabolomic analyses were performed on the samples. Our results demonstrated a significant age-related effect on the metabolomic profiles in both analyses. Specifically, post-hatchling turtles exhibited increased levels of urea ( < 0.001), triglyceride ( = 0.0003), cholesterol ( < 0.001), lysoPE (18:1/0:0) ( < 0.001), 7-HDoHE ( = 0.0121), pyrrolidinebutanoic acid ( < 0.001), formiminoglutamic acid ( < 0.001), pyroglutamic acid ( < 0.001), lysoPC (0:0/20:4) ( < 0.001), lysoPE (22:6/0:0) ( < 0.001), 1-acyl-sn-glycero-3-phosphocholine ( < 0.001), DL-homocysteine ( < 0.001) and gamma-Glutamyltyrosine ( < 0.001). Conversely, post-hatchlings showed reduced levels of total protein ( < 0.001), glucose ( = 0.0002), uric acid ( < 0.001), inorganic phosphorus ( = 0.0018) and calcium ( = 0.0410) compared with juveniles. These findings suggest significant physiological changes between the age groups, likely due to differentiated feeding patterns. Further research is needed to better understand the metabolic profiles and complex physiological and nutritional interactions of this species.
为了研究年龄对蠵龟代谢组学特征的影响，本研究分析了来自地中海沿岸不同地点的两个年龄组（50只刚孵化的幼龟和50只幼龟）的100份血浆样本。对样本进行了靶向和非靶向代谢组学分析。我们的结果表明，在这两种分析中，代谢组学特征都存在与年龄相关的显著影响。具体而言，刚孵化的幼龟尿素（P<0.001）、甘油三酯（P = 0.0003）、胆固醇（P<0.001）、溶血磷脂酰乙醇胺（18:1/0:0）（P<0.001）、7-羟基二十二碳六烯酸（P = 0.0121）、吡咯烷丁酸（P<0.001）、亚胺甲基谷氨酸（P<0.001）、焦谷氨酸（P<0.001）、溶血磷脂酰胆碱（0:0/20:4）（P<0.001）、溶血磷脂酰乙醇胺（22:6/0:0）（P<0.001）、1-酰基-sn-甘油-3-磷酸胆碱（P<0.001）、DL-高半胱氨酸（P<0.001）和γ-谷氨酰酪氨酸（P<0.001）水平升高。相反，与幼龟相比，刚孵化的幼龟总蛋白（P<0.001）、葡萄糖（P = 0.0002）、尿酸（P<0.001）、无机磷（P = 0.0018）和钙（P = 0.0410）水平降低。这些发现表明不同年龄组之间存在显著的生理变化，可能是由于不同的摄食模式所致。需要进一步研究以更好地了解该物种的代谢特征以及复杂的生理和营养相互作用。
