Chen Chung-Yu, Wang Wei-Jan, Wu Chen-Shiou, Wang Shao-Chun, Chang Wei-Chao, Hung Mien-Chie
Research Center for Cancer Biology, China Medical University Taichung 406040, Taiwan.
Department of Biological Science and Technology, China Medical University Taichung 406040, Taiwan.
Am J Cancer Res. 2024 May 15;14(5):2555-2569. doi: 10.62347/QJBG3026. eCollection 2024.
The COVID-19 pandemic has caused hundreds million cases and millions death as well as continues to infect human life in the world since late of 2019. The breakthrough infection caused from mutation of SARS-CoV-2 is rising even the vaccinated population has been increasing. Currently, the severe threat posed by SARS-CoV-2 has been alleviated worldwide, and the situation has transitioned to coexisting with the virus. The dietary food with antiviral activities may improve to prevent virus infection for living with COVID-19 pandemic. Teas containing enriched phenolic ingredients such as tannins have been reported to be antitumor agents as well as be good inhibitors for coronavirus. This study developed a highly sensitive and selective ultra-high performance liquid chromatography-high resolution mass spectrometric method for quantification of tannic acids, a hydrolysable tannin, and proanthocyanidins, a condense tannin, in teas with different levels of fermentation. The in vitro pseudoviral particles (Vpp) infection assay was used to evaluate the inhibition activities of various teas. The results of current research demonstrate that the tannins in teas are effective inhibitors against infection of SARS-CoV-2 and its variants.
自2019年末以来，新冠疫情已导致数亿例感染和数百万例死亡，且仍在持续影响全球人类生活。即便接种疫苗的人群不断增加，由新冠病毒变异导致的突破性感染仍在上升。目前，新冠病毒在全球构成的严重威胁已有所缓解，形势已转变为与病毒共存。具有抗病毒活性的膳食可能有助于预防病毒感染，以便在新冠疫情期间生存。据报道，含有丰富酚类成分（如单宁）的茶不仅是抗肿瘤剂，也是冠状病毒的良好抑制剂。本研究开发了一种高灵敏度和选择性的超高效液相色谱-高分辨率质谱法，用于定量不同发酵程度茶叶中的水解单宁——鞣酸和缩合单宁——原花青素。体外伪病毒颗粒（Vpp）感染试验用于评估各种茶叶的抑制活性。当前的研究结果表明，茶叶中的单宁是针对新冠病毒及其变体感染的有效抑制剂。
